Hyphenated Centrifugal Microsolid Phase Extraction and Centrifugal Acceleration Thin-Layer Chromatography in a Single Device: An Innovative Approach to Perform Consecutive Extraction and Separation

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-30 DOI:10.1021/acs.analchem.4c04252
Arezoo Eskandari, Reyhaneh Namdari, Shahram Seidi
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Abstract

An innovative approach for extracting and separating analytes consecutively from complex matrixes was introduced for the first time. The approach involves applying centrifugal force to a novel circular chip to expedite the process of microsolid phase extraction and centrifugal acceleration thin-layer chromatography, followed by red–green–blue (RGB) analysis as a simple, fast, and in situ detection method. Furthermore, the natural product of rice husk was utilized to create a sorbent with great adsorption capabilities. In this method, curcumin and phenazopyridine were employed as model drugs. The one-variable-at-a-time (OVAT) method was used to study the factors that affect the extraction and separation. Under the optimized conditions, a good relationship was observed between the |R| + |B| intensity and the concentration of analytes within the range of 1.0–10.0 μg/mL (R2 = 0.9903) for curcumin and 0.1–7.5 μg/mL (R2 = 0.9933) for phenazopyridine in urine samples. Intra- and interday RSDs% (n = 3) were obtained at less than 5.6 and 7.1%, respectively. In urine samples, the limit of detection values was 0.75 μg/mL for curcumin and 0.05 μg/mL for phenazopyridine, and relative recovery values were in the acceptable range of 85.0–100.2%. The suggested approach has the appropriate sensitivity, good accuracy, and acceptable applicability to determine curcumin and phenazopyridine in complicated matrices.

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分离式离心微固相萃取和离心加速薄层色谱:一种连续萃取分离的创新方法
首次提出了一种从复杂基质中连续提取和分离分析物的新方法。该方法通过对新型圆形芯片施加离心力来加快微固相萃取和离心加速薄层色谱的过程,然后进行红绿蓝(RGB)分析,作为一种简单,快速的原位检测方法。此外,还利用稻壳的天然产物制备了具有良好吸附性能的吸附剂。本方法以姜黄素和非那唑吡啶为模型药物。采用单变量一次法(OVAT)研究了影响提取分离的因素。在优化条件下,尿液样品中姜黄素浓度在1.0 ~ 10.0 μg/mL (R2 = 0.9903)范围内,苯那唑吡啶浓度在0.1 ~ 7.5 μg/mL (R2 = 0.9933)范围内,|R| + |B|强度与分析物浓度呈良好的相关关系。日内和日内rsd % (n = 3)分别小于5.6%和7.1%。尿液样品中姜黄素的检出限为0.75 μg/mL,非那唑吡啶的检出限为0.05 μg/mL,相对回收率在85.0 ~ 100.2%的可接受范围内。该方法对复杂基质中姜黄素和非那唑吡啶的测定具有适当的灵敏度、良好的准确性和可接受的适用性。
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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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