{"title":"Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii","authors":"Patricia Orlandi Barth MSc , Dariane Castro Pereira PhD , Gabriela Simões de Oliveira MSc , Loriane Rita Konkewicz MSc , Larissa Lutz PhD , William Latosinski Matos , Mariana Preussler Mott PhD , Caroline Collioni Constante MSc , Camila Morschbacher Wilhelm PhD , Laura Czekster Antochevis MSc , Rodrigo Minuto Paiva PhD , Kellen Figueira Tragnago , Afonso Luis Barth PhD , Andreza Francisco Martins PhD","doi":"10.1016/j.ajic.2024.11.019","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Acinetobacter seifertii</em>, a member of <em>A baumannii-calcoaceticus</em> complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant <em>A seifertii</em> among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.</div></div><div><h3>Methods</h3><div>All patients with carbapenem-resistant <em>A seifertii</em> diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (<em>bla</em><sub>IMP</sub>, <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>NDM</sub>, <em>bla</em><sub>KPC</sub>, <em>bla</em><sub>GES</sub>, <em>bla</em><sub>OXA-48-like</sub>, and <em>bla</em><sub>OXA-23</sub>) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.</div></div><div><h3>Results</h3><div>Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the <em>bla</em><sub>NDM-1</sub> gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the <em>bla</em><sub>NDM-1</sub> was carried by the same plasmid type.</div></div><div><h3>Discussion</h3><div>Our study has identified a novel strain of <em>A seifertii</em> carrying <em>bla</em><sub>NDM-1</sub> responsible for the outbreak, indicating its emergence in the institution.</div></div><div><h3>Conclusions</h3><div>This is the first report of carbapenem-resistant <em>A seifertii</em> ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant <em>A baumannii</em>, which is crucial in outbreak scenarios.</div></div>","PeriodicalId":7621,"journal":{"name":"American journal of infection control","volume":"53 4","pages":"Pages 473-478"},"PeriodicalIF":2.4000,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of infection control","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0196655324008599","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
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Abstract
Background
Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.
Methods
All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC, blaGES, blaOXA-48-like, and blaOXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.
Results
Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the blaNDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the blaNDM-1 was carried by the same plasmid type.
Discussion
Our study has identified a novel strain of A seifertii carrying blaNDM-1 responsible for the outbreak, indicating its emergence in the institution.
Conclusions
This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.
期刊介绍：
AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)
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