Effects of Maohuoside A on AMPK Activation in Rats with Osteoarthritis Induced by Anterior Cruciate Ligament Transection.

IF 1.7 4区医学 Q4 NEUROSCIENCES Journal of musculoskeletal & neuronal interactions Pub Date : 2024-12-01

Hongwei Wang

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引用次数: 0

Abstract

Objective: To explore the therapeutic effects of Maohuoside A (MHA) on rats with osteoarthritis (OA) induced by anterior cruciate ligament transection, and to analyze its impact on the activation of adenosine monophosphate-activated protein kinase (AMPK).

Methods: Sprague-Dawley male rats were divided into six groups: Sham, OA, L-MHA, M-MHA, H-MHA, and H-MHA + Compound C (n = 12 per group). The Sham group had sham surgery, while the OA, L-MHA, M-MHA, and H-MHA groups underwent OA via ACL severing. L-MHA, M-MHA, and H-MHA groups received low, moderate, and high doses of Maohuoside A, respectively, and the H-MHA + Compound C group received high-dose Maohuoside A plus Compound C. Hematoxylin-eosin (HE) staining and Safranin O/Fast Green staining were used to assess articular cartilage damage and degeneration, and the OARSI score was used to evaluate the extent of cartilage degeneration.

Results: Compared with the OA group, the L-MHA, M-MHA, and H-MHA groups showed decreased OARSI scores, increased relative levels of Bcl-2 mRNA, decreased relative levels of Bax mRNA, reduced serum levels of IL-1β, IL-6, and TNF-α, increased relative levels of Collagen II mRNA, decreased relative levels of MMP-13 mRNA, and increased relative levels of BMP2, Runx2, and Osterix mRNA. Additionally, the relative level of cartilage AMPKα (Thr172) phosphorylation increased (P<0.05).

Conclusion: This study suggests that Maohuoside A exerts anti-osteoarthritis (OA) effects by activating AMPK.

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毛藿香苷A对前交叉韧带断裂所致骨关节炎大鼠AMPK活化的影响。

目的：探讨毛藿香苷A （MHA）对前交叉韧带断裂所致骨关节炎（OA）大鼠的治疗作用，并分析其对单磷酸腺苷活化蛋白激酶（AMPK）活化的影响。方法：将Sprague-Dawley雄性大鼠分为Sham、OA、L-MHA、M-MHA、H-MHA、H-MHA +化合物C 6组，每组12只。Sham组采用假手术治疗，OA组、L-MHA组、M-MHA组和H-MHA组通过ACL切断OA。L-MHA、M-MHA、H-MHA组分别给予低、中、高剂量毛火花苷A， H-MHA +化合物C组给予高剂量毛火花苷A +化合物C。采用苏木精-伊红（HE）染色和红花素O/Fast Green染色评估关节软骨损伤和退变，采用OARSI评分评估软骨退变程度。结果：与OA组比较，L-MHA、M-MHA、H-MHA组OARSI评分降低，Bcl-2 mRNA相对水平升高，Bax mRNA相对水平降低，血清IL-1β、IL-6、TNF-α水平降低，Collagen II mRNA相对水平升高，MMP-13 mRNA相对水平降低，BMP2、Runx2、Osterix mRNA相对水平升高。此外，软骨AMPKα (Thr172)磷酸化的相对水平升高(p)。结论：本研究提示毛藿香苷A通过激活AMPK发挥抗骨关节炎（OA）的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of musculoskeletal & neuronal interactions NEUROSCIENCES-PHYSIOLOGY

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Musculoskeletal and Neuronal Interactions (JMNI) is an academic journal dealing with the pathophysiology and treatment of musculoskeletal disorders. It is published quarterly (months of issue March, June, September, December). Its purpose is to publish original, peer-reviewed papers of research and clinical experience in all areas of the musculoskeletal system and its interactions with the nervous system, especially metabolic bone diseases, with particular emphasis on osteoporosis. Additionally, JMNI publishes the Abstracts from the biannual meetings of the International Society of Musculoskeletal and Neuronal Interactions, and hosts Abstracts of other meetings on topics related to the aims and scope of JMNI.