Renshen (Radix Ginseng) polysaccharide promotes repair of the mice intestinal mucosa through regulatory mechanisms based on polyamine and human antigen R.

Abstract

Objective: To investigate the mechanism and effect of Renshen (Radix Ginseng) polysaccharide on the migration of intestinal epithelial cell line 6 (IEC-6), as well as the repair mechanism of Renshen (Radix Ginseng) polysaccharide on colonic injury induced by dextran sulfate sodium (DSS) in mice.

Methods: Mice were fed 3% (w/v) DSS for 6 d to create colonic lesions. A cell-migration model was created using cell scratching. mRNA expression, protein expression, translation efficiency of mRNA, and nucleoplasmic distribution of human antigen R (HuR) were determined by real-time reverse transcription-quantitative polymerase chain reaction, western blotting, a dual luciferase reporter system, and immunofluorescence staining, respectively.

Results: Renshen (Radix Ginseng) polysaccharide promoted the migration of IEC-6 cells and affected expression of stromal interaction molecule 1 (STIM1) and cell division cycle 42 (Cdc42) at transcriptional and post-transcriptional levels.

Conclusions: Renshen (Radix Ginseng) polysaccharide-induced repair of intestinal mucosal injury may be mediated by increased cell migration via polyamine-based regulatory mechanisms. In vitro and in vivo experiments suggest that Renshen (Radix Ginseng) polysaccharide-induced post-transcriptional regulation of STIM1 and Cdc42 may be related to differences in the regulation of different target genes by HuR. Taken together, these data provide a reference for further exploration of the protective effect of Renshen (Radix Ginseng) on the intestinal mucosa.