Fractal analysis to assess the differentiation state of oligodendroglia in culture.

IF 2.7 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Neuroscience Methods Pub Date : 2024-12-07 DOI:10.1016/j.jneumeth.2024.110336

J Pascual-Guerra, M Torres-Rico, C L Paíno, J A Rodríguez-Navarro, A G García

{"title":"Fractal analysis to assess the differentiation state of oligodendroglia in culture.","authors":"J Pascual-Guerra, M Torres-Rico, C L Paíno, J A Rodríguez-Navarro, A G García","doi":"10.1016/j.jneumeth.2024.110336","DOIUrl":null,"url":null,"abstract":"Background: Oligodendroglial development is accompanied by increased cell complexity. A simple and cost-effective evaluation of the pro-myelinating activity of different drugs and/or treatments would be of great interest. In cultured oligodendroglia, an evaluation of the pro-myelinating activity of different drugs and/or treatments can be achieved through fractal analysis, which allows measuring cell complexity.New method: Fractal dimension was assessed in two O4+ cell types (neural stem cell-derived and lineage-converted adipose tissue mesenchymal cells) under proliferating or differentiating conditions.Comparison with existing methods: This analysis, which was originally developed to analyze microglia, assigns a quantitative value (fractal dimension) to cellular profiles, obtaining higher coefficients as cells increase in size and arborizations instead of mRNA or protein quantification of mature oligodendroglial markers, such as MBP, MAG, O1 or PLP1/DM20.Results: This article describes a methodology to perform fractal analysis in immunofluorescent images of O4-positive (O4+) oligodendroglia using the FracLac plugin of ImageJ software. Pro-myelinating drug Benztropine-treated O4+ cells exhibit higher fractal dimension than control group.Conclusions: The results demonstrated the effectiveness and sensitivity of the fractal dimension coefficient provided by FracLac software to assess the effects of treatments on oligodendroglial differentiation.","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":" ","pages":"110336"},"PeriodicalIF":2.7000,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Neuroscience Methods","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jneumeth.2024.110336","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Oligodendroglial development is accompanied by increased cell complexity. A simple and cost-effective evaluation of the pro-myelinating activity of different drugs and/or treatments would be of great interest. In cultured oligodendroglia, an evaluation of the pro-myelinating activity of different drugs and/or treatments can be achieved through fractal analysis, which allows measuring cell complexity.

New method: Fractal dimension was assessed in two O4⁺ cell types (neural stem cell-derived and lineage-converted adipose tissue mesenchymal cells) under proliferating or differentiating conditions.

Comparison with existing methods: This analysis, which was originally developed to analyze microglia, assigns a quantitative value (fractal dimension) to cellular profiles, obtaining higher coefficients as cells increase in size and arborizations instead of mRNA or protein quantification of mature oligodendroglial markers, such as MBP, MAG, O1 or PLP1/DM20.

Results: This article describes a methodology to perform fractal analysis in immunofluorescent images of O4-positive (O4⁺) oligodendroglia using the FracLac plugin of ImageJ software. Pro-myelinating drug Benztropine-treated O4⁺ cells exhibit higher fractal dimension than control group.

Conclusions: The results demonstrated the effectiveness and sensitivity of the fractal dimension coefficient provided by FracLac software to assess the effects of treatments on oligodendroglial differentiation.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.