Analysis of microvascular density and collagen structure of the medial meniscotibial ligament of the knee: Immunohistochemical study with CD31 and histology with Picrosirius red

IF 1.6 4区医学 Q3 ORTHOPEDICS Knee Pub Date : 2024-12-06 DOI:10.1016/j.knee.2024.11.016

Amanda Progenio dos Santos , Jonatas Brito de Alencar Neto , Pedro Henrique Marques Amorim , Paulo Goberlânio de Barros Silva , Maria Luzete Costa Cavalcante

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Abstract

Background

The aim of the present study was to describe the structure of the collagen matrix and the microvascularization of the medial meniscotibial ligament (MMTL), in order to contribute to the refinement of the surgical technique for repairing these structures and consequently lead to a reduction in the risk of anterior cruciate ligament re-rupture.

Methods

Twelve MMTLs were obtained from deceased organ donors. The ligaments were initially analyzed macroscopically and evaluated histologically using hematoxylin and eosin staining. The evaluation of the collagen matrix was carried out using the Picrosirius red staining method under a polarized light microscope. Immunohistochemical study using monoclonal antibodies against CD31 was used to evaluate microvascularization.

Results

The MMTL was identified in 100% of the dissections. The hematoxylin and eosin-stained histological sections exhibited oriented and parallel collagen fibers, with low cellularity in its intrasubstance portion and increase in cellularity close to the meniscal insertion. Quantitative analysis of the collagen matrix showed a ratio of type I to type III collagen of 3.86 (±3.07). The average microvascular density in the intrasubstance portion was 33.71 ± 8.7 vessels/mm² and in the meniscal insertion portion it was 74.14 ± 23.85 vessels/mm².

Conclusion

The MMTL is a structure with a predominance of Type I collagen in relation to Type III collagen and high microvascular density in the region of meniscal insertion. These findings suggest that the healing potential of MMTL, regarding ramp injuries, is high because these injuries occur in the meniscal insertion area.

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膝关节内侧半月板韧带微血管密度及胶原结构分析：CD31免疫组化及小天狼星红组织学研究。

背景：本研究的目的是描述胶原基质的结构和内侧半月板胫韧带（MMTL）的微血管化，以促进修复这些结构的手术技术的改进，从而降低前交叉韧带再破裂的风险。方法：从死者器官供者中获得12个mmtl。韧带最初进行宏观分析，并用苏木精和伊红染色进行组织学评估。在偏光显微镜下，采用Picrosirius红染色法对胶原基质进行评价。采用CD31单克隆抗体免疫组化方法评价微血管形成。结果：MMTL的鉴别率为100%。苏木精和伊红染色的组织学切片显示胶原纤维取向平行，其实质部分细胞密度低，靠近半月板插入处细胞密度增加。胶原基质定量分析显示，I型与III型胶原的比例为3.86（±3.07）。物质内部分微血管密度平均值为33.71±8.7支/mm2，半月板止点部分微血管密度平均值为74.14±23.85支/mm2。结论：MMTL是一种I型胶原相对于III型胶原占优势的结构，在半月板止点区域微血管密度高。这些发现表明，对于斜坡损伤，MMTL的愈合潜力很高，因为这些损伤发生在半月板插入区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Knee 医学-外科

CiteScore

3.80

自引率

5.30%

发文量

171

审稿时长

6 months

期刊介绍： The Knee is an international journal publishing studies on the clinical treatment and fundamental biomechanical characteristics of this joint. The aim of the journal is to provide a vehicle relevant to surgeons, biomedical engineers, imaging specialists, materials scientists, rehabilitation personnel and all those with an interest in the knee. The topics covered include, but are not limited to: • Anatomy, physiology, morphology and biochemistry; • Biomechanical studies; • Advances in the development of prosthetic, orthotic and augmentation devices; • Imaging and diagnostic techniques; • Pathology; • Trauma; • Surgery; • Rehabilitation.