Generation of human induced pluripotent stem cell lines (iPSC) from adipose-derived mesenchymal stromal cells from two patients with systemic sclerosis.
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引用次数: 0
Abstract
Systemic sclerosis (SSc) is a rare and complex connective tissue disease associated with high morbidity and mortality. SSc is characterized by ischemic vasculopathy, cutaneous and visceral fibrosis and a dysimmune state (Denton and Khanna, 2017; Volkmann et al., 2023; Barnes and Mayes, 2012). We have derived induced pluripotent stem cell (iPSC) lines from two SSc patients aged 38 and 67 years with severe vascular phenotype. These iPSC lines expressed pluripotent markers, exhibited normal and stable genome, and differentiated into trilineage embryonic layers in teratoma formation assays. These SSc-specific iPSC lines can be differentiated into endothelial cells, providing a valuable model to elucidate vascular dysfunction and develop personalized therapeutic approaches.
系统性硬化症（SSc）是一种罕见和复杂的结缔组织疾病，具有高发病率和死亡率。SSc的特征是缺血性血管病变、皮肤和内脏纤维化以及免疫功能障碍(Denton and Khanna, 2017；Volkmann et al., 2023；Barnes and Mayes, 2012)。我们从两名年龄分别为38岁和67岁的严重血管表型SSc患者身上获得了诱导多能干细胞（iPSC）系。在畸胎瘤形成实验中，这些iPSC系表达多能性标记，表现出正常和稳定的基因组，并分化为三龄胚胎层。这些ssc特异性iPSC系可以分化为内皮细胞，为阐明血管功能障碍和开发个性化治疗方法提供了有价值的模型。
期刊介绍：
Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.
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