Biocontrol potential of natamycin-producing Streptomyces lydicus JCK-6019 against soil-borne fungal diseases of cucumber and characterization of its biocontrol mechanism

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-12-10 DOI:10.1002/ps.8596

Feng Luo, Hanna Gwak, Ae Ran Park, Van Thi Nguyen, Jin-Cheol Kim

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Abstract

BACKGROUND

Fusarium oxysporum f. sp. cucumerinum and Rhizoctonia solani AG-4 are the two most important fungal pathogens causing soil-borne fungal diseases of cucumber; they are difficult to control and cause serious economic losses. Given the detrimental effects of the indiscriminate use of chemical fungicides, biocontrol emerges as an efficient and ecofriendly alternative for managing soil-borne fungal diseases.

RESULTS

Streptomyces lydicus JCK-6019 (hereafter, JCK-6019) was isolated from rhizosphere soil. Its fermentation filtrate and volatile organic compounds exhibited broad-spectrum antifungal activity against various phytopathogenic fungi and oomycetes. JCK-6019 produced natamycin as an agar-diffusible antifungal metabolite. It also produced indole-3-acetic acid and various hydrolytic enzymes. In vivo experiments revealed that a ten-fold-diluted optimized JCK-6019 fermentation broth exhibited 100% control efficiency against cucumber damping-off disease and 62.5% control efficiency against cucumber Fusarium wilt disease. Pretreatment of cucumber seedlings with 1000-fold-diluted optimized JCK-6019 fermentation broth resulted in 68.18% and 23.91% disease control values against cucumber damping-off and Fusarium wilt disease, respectively. Moreover, peroxidase activity in cucumbers after 1 day of treatment was 1.5-fold higher than that in the control. Similarly, polyphenol oxidase activity in cucumbers after 3 days of treatment was 2.34-fold higher than that in the control, indicating that JCK-6019 can induce plant resistance.

CONCLUSION

The natamycin-producing strain JCK-6019 could effectively suppress the development of cucumber Fusarium wilt and damping-off disease by inducing plant resistance and producing antifungal metabolites, including natamycin and volatile organic compounds. Thus, JCK-6019 possesses high potential for application in the development of biocontrol agents against soil-borne fungal diseases of cucumber. © 2024 Society of Chemical Industry.

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产纳他霉素链霉菌JCK-6019对黄瓜土传真菌病害的生物防治潜力及其生物防治机制表征

黄瓜尖孢镰刀菌和黄瓜根丝核菌AG-4是引起黄瓜土传真菌病的两种最重要的真菌病原体；它们难以控制，造成严重的经济损失。鉴于滥用化学杀菌剂的有害影响，生物防治成为管理土传真菌病的一种有效和生态友好的替代办法。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.