An Experimental Insight Into the Role of Agomelatine in Renal Ischemia/Reperfusion Injury

IF 3.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Biochemical and Molecular Toxicology Pub Date : 2024-12-10 DOI:10.1002/jbt.70090
Damla Aykora, Mehmet Refik Bahar, Kevser Tanbek, Dilara Altay Öztürk, Elif Karaca, Süleyman Sandal, Suat Tekin
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Abstract

Acute kidney injury (AKI) is one of the leading causes of chronic kidney disease and accounts for 50%–75% of mortality following renal pathologies or organ transplantation. Ischemia‒reperfusion injury (IRI) involves an interrupted blood supply to organs and the kidney; IRI exacerbates AKI development. Owing to several pharmacological treatment methods, AKI still has a poor prognosis, and novel therapeutic options are needed. Agomelatine (AGM) is a melatonin receptor agonist (MT1 and MT2) with increased bioavailability and lipophilicity. In this study, we aimed to investigate the antioxidant and anti-inflammatory effects of AGM in experimental renal IRI via long-term and short-term applications. Sixty male Sprague–Dawley rats were randomly divided into six groups (n = 10): the control, I/R, AGM20S, AGM40S, AGM20L, and AGM40L groups. Following the establishment of the renal IRI model, the rats received agomelatine at 20 and 40 mg/kg orally, and agomelatine solvent (hydroxyethylcellulose) was used as a vehicle. At the end of the experiment, blood samples and renal tissues were harvested for histopathological and biochemical analysis. Urea, creatinine, tumor necrosis factor (TNF-α), and interleukin-1 beta (IL-1β) levels were measured in blood serum samples. Malondialdehyde (MDA) levels and increased superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), and total glutathione (GSH) levels were measured in renal tissue supernatants. Our biochemical results indicated that AGM reduced creatinine, TNF-α, IL-1β, and malondialdehyde levels and increased SOD, CAT, GSHPx, and total GSH levels. Agolematine reduced infiltration, intratubular hemorrhage, and intratubular cast formation histopathologically. Our results suggest that AGM could be a potential therapeutic adjuvant agent for ischemia‒reperfusion injury in the kidney and several other organs.

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急性肾损伤(AKI)是慢性肾病的主要病因之一,占肾脏病变或器官移植后死亡率的 50%-75%。缺血再灌注损伤(IRI)是指器官和肾脏的供血中断;IRI 会加剧 AKI 的发展。虽然有多种药物治疗方法,但 AKI 的预后仍然很差,因此需要新的治疗方案。阿戈美拉汀(AGM)是一种褪黑素受体激动剂(MT1 和 MT2),具有更高的生物利用度和亲油性。在这项研究中,我们旨在通过长期和短期应用,研究 AGM 在实验性肾脏 IRI 中的抗氧化和抗炎作用。60 只雄性 Sprague-Dawley 大鼠被随机分为 6 组(n = 10):对照组、I/R 组、AGM20S 组、AGM40S 组、AGM20L 组和 AGM40L 组。建立肾脏 IRI 模型后,大鼠分别口服 20 和 40 mg/kg 的阿戈美拉汀,并使用阿戈美拉汀溶剂(羟乙基纤维素)作为载体。实验结束后,采集血液样本和肾组织进行组织病理学和生化分析。测量血清样本中的尿素、肌酐、肿瘤坏死因子(TNF-α)和白细胞介素-1β(IL-1β)水平。在肾组织上清液中测量了丙二醛(MDA)水平和增加的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSHPx)和总谷胱甘肽(GSH)水平。生化结果表明,AGM 降低了肌酐、TNF-α、IL-1β 和丙二醛水平,提高了 SOD、CAT、GSHPx 和总 GSH 水平。从组织病理学角度看,阿戈美汀可减少浸润、管腔内出血和管腔内铸型的形成。我们的研究结果表明,AGM 可能是治疗肾脏和其他器官缺血再灌注损伤的潜在辅助药物。
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来源期刊
CiteScore
5.80
自引率
2.80%
发文量
277
审稿时长
6-12 weeks
期刊介绍: The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
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