Cilostazol geno-protective effects mitigate carbamazepine-induced genotoxicity in human cultured blood lymphocytes.

Q1 Environmental Science Toxicology Reports Pub Date : 2024-11-17 eCollection Date: 2024-12-01 DOI:10.1016/j.toxrep.2024.101814

Enaam M Al Momany, Abeer M Rababa'h, Karem H Alzoubi, Omar F Khabour

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Abstract

Background: Carbamazepine is one of the most widely used antiepileptic drugs. Carbamazepine has been shown to be toxic to cells. Cilostazol, an antiplatelet agent, has known antioxidant, antiproliferative, anti-inflammatory, and anti-tumor effects.

Objective: This study aimed to explore whether carbamazepine and cilostazol exert genotoxic and/or cytotoxic effects in human cultured blood lymphocytes and the impact of combining both drugs on such effects.

Methods: Genotoxicity was examined using sister chromatid exchange (SCE) assay, while cytotoxicity was evaluated by cell kinetic assays (mitotic and proliferative indices).

Results: Study findings have revealed that carbamazepine markedly increased SCEs (p<0.01), while cilostazol significantly decreased their frequencies (p<0.01). In addition, the frequency of SCEs of the combination of both drugs was similar to that of the control group (p>0.05). Carbamazepine increased the cell proliferative index (p<0.01) while cilostazol decreased it (p<0.01). The proliferative index was normalized to the control level when both drugs were combined.

Conclusion: We suggest that cilostazol has the potential to protect human lymphocytes from carbamazepine-induced toxic effects.

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西洛他唑的基因保护作用减轻卡马西平诱导的人培养血液淋巴细胞的遗传毒性。

背景：卡马西平是应用最广泛的抗癫痫药物之一。卡马西平已被证明对细胞有毒。西洛他唑是一种抗血小板药物，具有抗氧化、抗增殖、抗炎和抗肿瘤作用。目的：本研究旨在探讨卡马西平和西洛他唑是否对人培养的血液淋巴细胞具有遗传毒性和/或细胞毒性作用，以及两药联合使用对这种作用的影响。方法：采用姐妹染色单体交换法（SCE）检测遗传毒性，采用细胞动力学法（有丝分裂和增殖指标）评价细胞毒性。结果：研究结果显示卡马西平显著增加sce （p0.05）。结论：西洛他唑具有保护人淋巴细胞免受卡马西平毒性作用的作用。

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