Construction and validation of a survival prognostic model for clear cell renal cell carcinoma.

IF 1.1 4区 医学 Q3 UROLOGY & NEPHROLOGY Clinical nephrology Pub Date : 2025-03-01 DOI:10.5414/CN111509
Chen-Li Li, Yu-Qian Jiang, Wei Pan, Yan-Li Yang
{"title":"Construction and validation of a survival prognostic model for clear cell renal cell carcinoma.","authors":"Chen-Li Li, Yu-Qian Jiang, Wei Pan, Yan-Li Yang","doi":"10.5414/CN111509","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Utilizing expression data of clear cell renal cell carcinoma (ccRCC) genes from the Cancer Genome Atlas (TCGA) database, this study employs weighted gene co-expression network analysis (WGCNA) and Cox regression analysis to identify genes associated with the occurrence and development of ccRCC, thereby providing a scientific basis for its treatment.</p><p><strong>Materials and methods: </strong>Differentially expressed genes between tumor and control groups were identified by preprocessing and batch correction of ccRCC transcriptome data in the TCGA database using the Wilcoxon test. Prognostic prediction models were established through a combination of WGCNA analysis, univariate Cox regression analysis, and multivariate Cox regression analysis. The reliability of these prognostic models was evaluated by plotting Kaplan-Meier survival analysis and receiver operating characteristic (ROC) curves and by further analyzing the relationship between model gene expression levels, tumor staging, and tumor grading.</p><p><strong>Results: </strong>Post-batch correction, M2-type macrophage infiltration was pronounced in tumor tissue, and 13 out of 290 screened relevant differential genes were included in the prognostic model. The Kaplan-Meier survival curves indicated that the 3- and 5-year overall survival rates were significantly higher in the low-risk group compared with the high-risk group (83.7 vs. 69.1%; 75.7 vs. 52.6%, p = 1.169e-08). The area under the ROC curve was 0.732, signifying strong predictive power for the survival curve. In this model, the expression levels of 11 genes were positively correlated with tumor stage and pathological grade, whereas the remaining 2 genes were negatively correlated.</p><p><strong>Conclusion: </strong>This model can predict the overall survival of patients with ccRCC and has the potential to become an important therapeutic target.</p>","PeriodicalId":10396,"journal":{"name":"Clinical nephrology","volume":" ","pages":"200-212"},"PeriodicalIF":1.1000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical nephrology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5414/CN111509","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: Utilizing expression data of clear cell renal cell carcinoma (ccRCC) genes from the Cancer Genome Atlas (TCGA) database, this study employs weighted gene co-expression network analysis (WGCNA) and Cox regression analysis to identify genes associated with the occurrence and development of ccRCC, thereby providing a scientific basis for its treatment.

Materials and methods: Differentially expressed genes between tumor and control groups were identified by preprocessing and batch correction of ccRCC transcriptome data in the TCGA database using the Wilcoxon test. Prognostic prediction models were established through a combination of WGCNA analysis, univariate Cox regression analysis, and multivariate Cox regression analysis. The reliability of these prognostic models was evaluated by plotting Kaplan-Meier survival analysis and receiver operating characteristic (ROC) curves and by further analyzing the relationship between model gene expression levels, tumor staging, and tumor grading.

Results: Post-batch correction, M2-type macrophage infiltration was pronounced in tumor tissue, and 13 out of 290 screened relevant differential genes were included in the prognostic model. The Kaplan-Meier survival curves indicated that the 3- and 5-year overall survival rates were significantly higher in the low-risk group compared with the high-risk group (83.7 vs. 69.1%; 75.7 vs. 52.6%, p = 1.169e-08). The area under the ROC curve was 0.732, signifying strong predictive power for the survival curve. In this model, the expression levels of 11 genes were positively correlated with tumor stage and pathological grade, whereas the remaining 2 genes were negatively correlated.

Conclusion: This model can predict the overall survival of patients with ccRCC and has the potential to become an important therapeutic target.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
透明细胞肾细胞癌生存预后模型的构建与验证。
目的:利用肿瘤基因组图谱(Cancer Genome Atlas, TCGA)数据库中透明细胞肾细胞癌(clear cell renal cell carcinoma, ccRCC)基因的表达数据,采用加权基因共表达网络分析(weighted gene co-expression network analysis, WGCNA)和Cox回归分析,鉴定与ccRCC发生发展相关的基因,为其治疗提供科学依据。材料和方法:采用Wilcoxon检验对TCGA数据库中的ccRCC转录组数据进行预处理和批量校正,鉴定出肿瘤组与对照组之间的差异表达基因。通过WGCNA分析、单因素Cox回归分析和多因素Cox回归分析相结合建立预后预测模型。通过绘制Kaplan-Meier生存分析和受试者工作特征(ROC)曲线,并进一步分析模型基因表达水平、肿瘤分期和肿瘤分级之间的关系,评估这些预后模型的可靠性。结果:批校正后,肿瘤组织可见m2型巨噬细胞浸润,筛选的290个相关差异基因中有13个被纳入预后模型。Kaplan-Meier生存曲线显示,低危组的3年和5年总生存率明显高于高危组(83.7 vs 69.1%;75.7 vs. 52.6%, p = 1.169e-08)。ROC曲线下面积为0.732,生存曲线具有较强的预测能力。在该模型中,11个基因的表达水平与肿瘤分期和病理分级呈正相关,其余2个基因的表达水平呈负相关。结论:该模型可预测ccRCC患者的总生存期,有望成为重要的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Clinical nephrology
Clinical nephrology 医学-泌尿学与肾脏学
CiteScore
2.10
自引率
9.10%
发文量
138
审稿时长
4-8 weeks
期刊介绍: Clinical Nephrology appears monthly and publishes manuscripts containing original material with emphasis on the following topics: prophylaxis, pathophysiology, immunology, diagnosis, therapy, experimental approaches and dialysis and transplantation.
期刊最新文献
Standing on the shoulders of a giant. A national survey of pediatric nephrologists on the treatment of steroid-resistant nephrotic syndrome. Trends and surgical outcomes in laparoscopic and open peritoneal dialysis catheter insertion. Immunosuppressant-resistant nephrotic syndrome and primary amenorrhea: A case report of adult Frasier syndrome and literature review. The role of POCUS in sepsis-associated AKI in children.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1