Analysis of antibiotic susceptibility and genomic characteristics of Helicobacter pylori by whole-genome resequencing in Northern China.

IF 2.1 4区生物学 Q3 MICROBIOLOGY Brazilian Journal of Microbiology Pub Date : 2024-12-11 DOI:10.1007/s42770-024-01582-w

Yaxuan Wang, Tiantong Jiang, Xiaochuan Liu, Rina Sa, Xiaoyan Zhu, Jihong Hu

{"title":"Analysis of antibiotic susceptibility and genomic characteristics of Helicobacter pylori by whole-genome resequencing in Northern China.","authors":"Yaxuan Wang, Tiantong Jiang, Xiaochuan Liu, Rina Sa, Xiaoyan Zhu, Jihong Hu","doi":"10.1007/s42770-024-01582-w","DOIUrl":null,"url":null,"abstract":"Introduction: Helicobacter pylori (H. pylori) is a major human pathogen whose increased antibiotic resistance poses a serious threat to human health.Aim: The aim of this study is to further explore the association between H. pylori resistance to clarithromycin, levofloxacin, metronidazole, amoxicillin, rifampicin, tetracycline and its genomic characteristics.Methodology: Using H. pylori isolates, we studied their susceptibility to six antibiotics by the agar dilution method. By performing whole-genome resequencing of the H. pylori genomic DNA, the differences in single nucleotide polymorphisms (SNPs) between phenotype resistant and sensitive strains were statistically analyzed to identify potential mutation sites related to drug resistance, and the consistency between genotype and phenotype resistance was analyzed.Results: The drug resistance rates of 65 H. pylori isolates are as follows: clarithromycin 36.9%, levofloxacin 29.2%, metronidazole 63.1%, amoxicillin 7.7%, rifampicin 12.3%, and tetracycline 3.1%. Based on the whole genome resequencing results of H. pylori isolates, 10 new mutations that may be related to drug resistance were identified. There is strong consistency between the genotype and phenotype resistance of clarithromycin and levofloxacin.Conclusion: The resistance rate to amoxicillin and tetracycline is relatively low in Northern China. and the above two antibiotics can be given priority for clinical treatment. It has a high resistance rate to metronidazole and should be avoided as much as possible, or combined with other drugs for treatment. The 10 mutations identified through analysis that only exist in drug-resistant strains may be associated with levofloxacin, metronidazole, amoxicillin, and rifampicin resistance, respectively. The results indicate that genotype testing of H. pylori can serve as a method for predicting its resistance to clarithromycin and levofloxacin.","PeriodicalId":9090,"journal":{"name":"Brazilian Journal of Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s42770-024-01582-w","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Helicobacter pylori (H. pylori) is a major human pathogen whose increased antibiotic resistance poses a serious threat to human health.

Aim: The aim of this study is to further explore the association between H. pylori resistance to clarithromycin, levofloxacin, metronidazole, amoxicillin, rifampicin, tetracycline and its genomic characteristics.

Methodology: Using H. pylori isolates, we studied their susceptibility to six antibiotics by the agar dilution method. By performing whole-genome resequencing of the H. pylori genomic DNA, the differences in single nucleotide polymorphisms (SNPs) between phenotype resistant and sensitive strains were statistically analyzed to identify potential mutation sites related to drug resistance, and the consistency between genotype and phenotype resistance was analyzed.

Results: The drug resistance rates of 65 H. pylori isolates are as follows: clarithromycin 36.9%, levofloxacin 29.2%, metronidazole 63.1%, amoxicillin 7.7%, rifampicin 12.3%, and tetracycline 3.1%. Based on the whole genome resequencing results of H. pylori isolates, 10 new mutations that may be related to drug resistance were identified. There is strong consistency between the genotype and phenotype resistance of clarithromycin and levofloxacin.

Conclusion: The resistance rate to amoxicillin and tetracycline is relatively low in Northern China. and the above two antibiotics can be given priority for clinical treatment. It has a high resistance rate to metronidazole and should be avoided as much as possible, or combined with other drugs for treatment. The 10 mutations identified through analysis that only exist in drug-resistant strains may be associated with levofloxacin, metronidazole, amoxicillin, and rifampicin resistance, respectively. The results indicate that genotype testing of H. pylori can serve as a method for predicting its resistance to clarithromycin and levofloxacin.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

华北地区幽门螺杆菌的全基因组重测序及抗生素敏感性分析。

简介：幽门螺杆菌（Helicobacter pylori， H. pylori）是一种主要的人类病原体，其耐药性的增加对人类健康构成严重威胁。目的：本研究旨在进一步探讨幽门螺旋杆菌对克拉霉素、左氧氟沙星、甲硝唑、阿莫西林、利福平、四环素的耐药与基因组特征的关系。方法：以幽门螺旋杆菌分离株为研究对象，采用琼脂稀释法研究其对6种抗生素的敏感性。通过对幽门螺杆菌基因组DNA进行全基因组重测序，统计分析表型耐药菌株与敏感菌株单核苷酸多态性（snp）差异，发现与耐药相关的潜在突变位点，并分析基因型与表型耐药的一致性。结果：65株幽门螺旋杆菌的耐药率分别为克拉霉素36.9%、左氧氟沙星29.2%、甲硝唑63.1%、阿莫西林7.7%、利福平12.3%、四环素3.1%。根据幽门螺杆菌分离株全基因组重测序结果，鉴定出10个可能与耐药相关的新突变。克拉霉素和左氧氟沙星耐药基因型和表型具有较强的一致性。结论：中国北方地区对阿莫西林和四环素的耐药率较低。临床可优先使用上述两种抗生素。对甲硝唑耐药率高，应尽量避免，或与其他药物联合治疗。通过分析发现的仅存在于耐药菌株中的10个突变可能分别与左氧氟沙星、甲硝唑、阿莫西林和利福平耐药有关。结果表明，幽门螺杆菌基因型检测可作为预测其对克拉霉素和左氧氟沙星耐药的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

文献相关原料

公司名称

产品信息

索莱宝

sucrose

来源期刊

Brazilian Journal of Microbiology 生物-微生物学

CiteScore

4.10

自引率

4.50%

发文量

216

审稿时长

1.0 months

期刊介绍： The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.