Association of HLA-G 3′-UTR Haplotypes With Recurrent Spontaneous Abortion in Women From Northwest Iran

IF 1.6 4区医学 Q4 DEVELOPMENTAL BIOLOGY Birth Defects Research Pub Date : 2024-12-12 DOI:10.1002/bdr2.2418

Amin Moqadami, Mohammad Khalaj-Kondori, Mehdi Haghi

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Abstract

Background and Objective(s)

Human leukocyte antigen-G (HLA-G) is a critical protein in immune regulation and tolerance. Recurrent spontaneous abortion (RSA) is a complex disease influenced by genetic, immune dysfunction, and environmental factors. This study investigates the role of HLA-G polymorphisms in the development of RSA.

Methods

Blood samples were collected from 80 women with RSA and 200 women without a history of RSA. After DNA extraction, PCR was used to sequence the 3′-UTR region. Allelic and genotypic frequencies were analyzed, and Haploview software was used for haplotype analysis.

Results

Individual polymorphisms did not significantly differ between the two groups. However, haplotype analysis revealed significant differences. The UTR-2 haplotype was more frequent in the RSA group compared to the healthy control group (p = 0.020), suggesting a potential association. Conversely, the UTR-4 haplotype had a significantly lower frequency in the RSA group (p = 0.041), indicating a protective role against RSA.

Conclusions

While individual polymorphisms did not differ significantly, haplotype analysis identified significant associations with RSA. These findings provide valuable insights into the genetic basis of the disease and may contribute to the development of new treatments and diagnostic tools.

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hla - g3′-UTR单倍型与伊朗西北部妇女复发性自然流产的关系

背景和目的：人白细胞抗原- g （HLA-G）是免疫调节和耐受的关键蛋白。复发性自然流产（RSA）是一种受遗传、免疫功能障碍和环境因素影响的复杂疾病。本研究探讨HLA-G多态性在RSA发展中的作用。方法：采集80例RSA女性和200例无RSA病史女性的血液样本。提取DNA后，采用PCR对3’-UTR区进行测序。分析等位基因和基因型频率，采用Haploview软件进行单倍型分析。结果：两组间个体多态性无显著差异。然而，单倍型分析显示了显著差异。与健康对照组相比，RSA组的UTR-2单倍型更常见（p = 0.020），提示可能存在关联。相反，UTR-4单倍型在RSA组中出现的频率明显较低（p = 0.041），表明其对RSA具有保护作用。结论：虽然个体多态性没有显著差异，但单倍型分析确定了与RSA的显著关联。这些发现为了解该病的遗传基础提供了有价值的见解，并可能有助于开发新的治疗方法和诊断工具。

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来源期刊

Birth Defects Research Medicine-Embryology

CiteScore

3.60

自引率

9.50%

发文量

153

期刊介绍： The journal Birth Defects Research publishes original research and reviews in areas related to the etiology of adverse developmental and reproductive outcome. In particular the journal is devoted to the publication of original scientific research that contributes to the understanding of the biology of embryonic development and the prenatal causative factors and mechanisms leading to adverse pregnancy outcomes, namely structural and functional birth defects, pregnancy loss, postnatal functional defects in the human population, and to the identification of prenatal factors and biological mechanisms that reduce these risks. Adverse reproductive and developmental outcomes may have genetic, environmental, nutritional or epigenetic causes. Accordingly, the journal Birth Defects Research takes an integrated, multidisciplinary approach in its organization and publication strategy. The journal Birth Defects Research contains separate sections for clinical and molecular teratology, developmental and reproductive toxicology, and reviews in developmental biology to acknowledge and accommodate the integrative nature of research in this field. Each section has a dedicated editor who is a leader in his/her field and who has full editorial authority in his/her area.