Enhancing medicinal proteins production in plant bioreactors: A focal review on promoters.

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL Fitoterapia Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI:10.1016/j.fitote.2024.106338

Cai Xiaoqi, Ning Kang, Xu Pei

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Abstract

The use of plant bioreactors for the production of medicinal proteins has emerged as a promising and cost-effective alternative to traditional microbial and mammalian cell culture systems. This review provides a focused examination of the critical role of promoters in enhancing the production of therapeutic proteins within plant-based platforms. We discuss the latest advancements in promoter discovery, modification, and optimization for the expression of medicinal proteins in plants. The review highlights the challenges and opportunities associated with various types of promoters, including constitutive, tissue-specific, and inducible promoters, and their impact on medicinal protein yield and quality. Case studies are presented to illustrate the successful application of these promoter engineering techniques in plant bioreactors, emphasizing the potential for scalable and sustainable production of pharmaceutical proteins. Additionally, we explore the strategies for improving promoter function. This review is intended to guide researchers and industry professionals in the selection and design of promoters for the enhanced production of medicinal proteins in plant bioreactors.

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提高植物生物反应器中药用蛋白质的产量：关于促进剂的重点综述。

使用植物生物反应器生产药用蛋白质已成为一种替代传统微生物和哺乳动物细胞培养系统的前景广阔、成本效益高的方法。本综述重点探讨了启动子在提高基于植物平台的治疗蛋白生产中的关键作用。我们讨论了在植物中表达药用蛋白的启动子发现、修饰和优化方面的最新进展。综述强调了与各类启动子（包括组成型、组织特异性和诱导型启动子）相关的挑战和机遇，以及它们对药用蛋白产量和质量的影响。文章通过案例研究说明了这些启动子工程技术在植物生物反应器中的成功应用，强调了可扩展和可持续生产药用蛋白质的潜力。此外，我们还探讨了改善启动子功能的策略。本综述旨在指导研究人员和行业专业人员选择和设计启动子，以提高植物生物反应器中药用蛋白质的产量。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.