Seed-transmission of turnip mosaic virus demonstrated unequivocally in a Brassica species.

IF 4.4 2区 农林科学 Q1 PLANT SCIENCES Plant disease Pub Date : 2024-12-13 DOI:10.1094/PDIS-09-24-1981-SC
Nuraizat Abidin, Martin John Barbetti, Ming Pei You, Roger Anthony Charles Jones
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Abstract

Turnip mosaic virus (TuMV) causes important diseases in Brassicaceae crops worldwide. In 2023, Brassica rapa ssp. perviridis cv. Tendergreen seedlings with virus-like symptoms were found growing within an insect-proof glasshouse. The affected seed lot (A), three others of Tendergreen, and five belonging to other B. rapa subspecies, B. juncea or Wasabia japonica were sown in trays within Controlled Environment Rooms (CER's) from which other plants were absent. TuMV was detected in 10% of seed lot A seedlings but none from other seed lots. Next, seed lot A (experiment 1) or seed from Tendergreen mother plants infected with B. napus TuMV resistance breaking strain isolate 12.1 (experiment 2) were sown in trays. In each experiment, these trays were subdivided into two batches, one being placed inside transparent plastic boxes, each batch then being placed in different CER's. TuMV was detected in 10% and 9% of seedlings inside or outside the boxes (experiment 1), or 1% of seedlings from both situations (experiment 2). Since virus contamination by aphid vectors or contact was excluded, TuMV seed transmission was demonstrated unequivocally. A complete TuMV genome obtained from an infected seedling (isolate BRSB1, accession PQ160044) was compared with 44 other genomic sequences from TuMV phylogroup World-B. It belonged to the same subclade as Australian resistance breaking strain isolates 12.1 and 12.5 (99.9% nucleotide identities) and 10 New Zealand sequences (99.2-99.5% nucleotide identities). Our findings have important implications concerning sowing crops with TuMV-infected seed and spreading readily seed-borne or resistance breaking TuMV strains nationally or internationally.

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在一个芸苔属物种中明确证实了芜菁花叶病毒的种子传播。
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来源期刊
Plant disease
Plant disease 农林科学-植物科学
CiteScore
5.10
自引率
13.30%
发文量
1993
审稿时长
2 months
期刊介绍: Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.
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