Asiaticoside alleviated NAFLD by activating Nrf2 and inhibiting the NF-κB pathway

Abstract

Background

Nonalcoholic fatty liver disease (NAFLD) is a globally prevalent clinical problem of chronic liver disorder whose main pathogenic mechanisms are hepatic lipid accumulation, oxidative stress, and inflammatory reactions. Asiaticoside (Asi) is a compound derived from Centella asiatica, possesses antioxidant, antiphlogistic, antifibrotic, as well as wound healing properties.

Purpose

The aim of this study was to investigate the effects of Asi on NAFLD and its potential mechanisms.

Study design

Two versions of experimental models were constructed using free fatty acids (FFAs)-stimulated HepG2 cells along with high-fat diet (HFD)-incited NAFLD in mice.

Methods

The pivotal action of Nrf2 was then explored using Ml-385 or Nrf2^-/- mice.

Results

The results indicated that Asi activated the Nrf2 to alleviate oxidative stress, inhibited the NF-κB to reduce the inflammatory response, and notably decreased lipid droplets and alleviated steatosis.

Conclusion

In conclusion, Asi demonstrates a potential to activate Nrf2 as well as inhibit the NF-κB pathway, there alleviate NAFLD.