mRNA m6A detection

IF 50.1 Q1 MULTIDISCIPLINARY SCIENCES Nature reviews. Methods primers Pub Date : 2024-12-12 DOI:10.1038/s43586-024-00365-9

Sharon Moshitch-Moshkovitz, Michal Sevilla-Sharon, Reut Ashwal-Fluss, Efrat Glick-Saar, Gideon Rechavi, Dan Dominissini

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Abstract

N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions. N6-methyladenosine (m6A) is an mRNA modification influencing gene expression. Advanced methodologies for mapping m6A enhance understanding of its dynamic roles and interactions. In this Primer, Moshitch-Moshkovitz et al. discuss various detection tools and analysis techniques facilitating insights into RNA epitranscriptomics.

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