Novel dual-channel ratiometric fluorescence probe for SO2 detection in food and bioimaging applications based on FRET mechanism

IF 4.7 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioorganic Chemistry Pub Date : 2025-01-01 DOI:10.1016/j.bioorg.2024.108034

Shufei Li , Huiquan Xiao , Jiaxin Hong , Xinyu Xia , Luying Duan , Wuying Yang , Xiaoli Yin , Yunhua Zeng , Yanping Hong

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Abstract

SO₂ is commonly used to ensure the safety of food, but englobing of excessive SO₂ poses serious risks to human health. Additionally, as fourth gaseous signaling molecule, it plays a critical role in various physiological processes. Therefore, monitoring the concentration of SO₂ in food and cells is crucial for correlative research and disease diagnosis. Herein, two ratiometric fluorescence probes (EHDA and EHDB) have been synthesized for detecting sulfite based on fluorescence resonance energy transfer (FRET). Undoubtedly, EHDA as the preferable probe due to its superior discernibility to SO₃^2– than that of EHDB. Moreover, EHDA featured rapid response, high sensitivity, excellent selectivity, and low cytotoxicity in physiological pH. Besides, EHDA could be expanded to establish convenient smartphone SO₂ sensing platform through innovatively loading EHDA on polyvinylidene fluoride membrane. Fortunately, EHDA has been utilized for determining SO₃^2– in food samples and imaging the fluctuation of sulfite in living cells and zebrafish.

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基于 FRET 机制用于食品和生物成像应用中二氧化硫检测的新型双通道比率荧光探针。

二氧化硫通常用于保证食品安全，但过量的二氧化硫会对人体健康造成严重威胁。此外，作为第四个气体信号分子，它在各种生理过程中起着至关重要的作用。因此，监测食物和细胞中SO2的浓度对相关研究和疾病诊断具有重要意义。本文合成了两种基于荧光共振能量转移（FRET）的比例荧光探针EHDA和EHDB，用于检测亚硫酸盐。EHDA对SO32-的识别能力优于EHDB，无疑是首选探针。此外，EHDA在生理ph下具有快速响应、高灵敏度、高选择性和低细胞毒性等特点。此外，通过创新性地将EHDA加载到聚偏氟乙烯膜上，可将EHDA扩展为建立便捷的智能手机SO2传感平台。幸运的是，EHDA已被用于测定食品样品中的SO32-和成像活细胞和斑马鱼中亚硫酸盐的波动。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.