Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-12-01 DOI:10.1111/mpp.70042
Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang
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Abstract

The organs of a plant species vary in cell structure, metabolism and defence responses. However, the mechanisms that enable a single pathogen to colonise different plant organs remain unclear. Here we compared the transcriptome of the oomycete pathogen Phytophthora sojae during infection of roots versus leaves of soybeans. We found differences in the transcript levels of hundreds of pathogenicity-related genes, particularly genes encoding carbohydrate-active enzymes, secreted (effector) proteins, oxidoreductase-related proteins and transporters. To identify the key regulator for root-specific infection, we knocked out root-specific transcription factors (TFs) and found the mutants of PsBZPc29, which encodes a member of an oomycete-specific class of basic leucine zipper (bZIP) TFs, displayed reduced virulence on soybean roots but not on leaves. More than 60% of the root-specific genes showed reduced expression in the mutants during root infection. The results suggest that transcriptional regulation underlies the organ-specific infection by P. sojae, and that a bZIP TF plays a key role in root-specific transcriptional regulation.

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植物物种的器官在细胞结构、新陈代谢和防御反应方面各不相同。然而,单一病原体在不同植物器官中定植的机制仍不清楚。在这里,我们比较了卵菌病原体 Phytophthora sojae 在感染大豆根部和叶片时的转录组。我们发现数百个病原体相关基因的转录水平存在差异,尤其是编码碳水化合物活性酶、分泌(效应)蛋白、氧化还原酶相关蛋白和转运体的基因。为了确定根特异性感染的关键调节因子,我们敲除了根特异性转录因子(TFs),发现编码卵菌特异性基本亮氨酸拉链(bZIP)TFs 的 PsBZPc29 突变体对大豆根的毒力降低,但对叶的毒力没有降低。在根部感染期间,突变体中超过 60% 的根特异性基因表达量减少。结果表明,转录调控是 P. sojae 对器官特异性感染的基础,而 bZIP TF 在根特异性转录调控中起着关键作用。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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