Elevated hematopoietic stem cell frequency in mouse alveolar bone marrow.

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING Stem Cell Reports Pub Date : 2024-12-11 DOI:10.1016/j.stemcr.2024.11.004
Kouta Niizuma, Satoru Morikawa, Eric Gars, Jinyi Xiang, Tomoko Matsubara-Takahashi, Rei Saito, Eri Takematsu, Yuting Wang, Haojun Xu, Arata Wakimoto, Tze Kai Tan, Yoshiaki Kubota, Charles K F Chan, Irving L Weissman, Taneaki Nakagawa, Adam C Wilkinson, Hiromitsu Nakauchi, Ryo Yamamoto
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Abstract

Hematopoietic stem cells (HSCs) are crucial for maintaining hematopoietic homeostasis and are localized within distinct bone marrow (BM) niches. While BM niches are often considered similar across different skeletal sites, we discovered that the alveolar BM (al-BM) in the mandible harbors the highest frequency of immunophenotypic HSCs in nine different skeletal sites. Transplantation assays revealed significantly increased engraftment from al-BM compared to femur, tibia, or pelvis BM, likely due to a higher proportion of alveolar HSCs. Moreover, hematopoietic progenitor cells (c-Kit+ Sca-1+ Lin-) in al-BM exhibited increased quiescence and reduced apoptosis, indicating superior maintenance and survival characteristics. We also observed an enrichment of mesenchymal stromal cells and skeletal stem cells in al-BM, suggesting a more supportive microenvironment. These findings indicate that al-BM provides a unique microenvironment conducive to higher frequency of HSCs, offering new insights into site-specific hematopoiesis.

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造血干细胞(HSCs)是维持造血平衡的关键,它们分布在不同的骨髓(BM)龛位中。虽然不同骨骼部位的骨髓龛通常被认为是相似的,但我们发现,在九个不同的骨骼部位中,下颌骨的肺泡骨髓(al-BM)蕴藏着最高频率的免疫分型造血干细胞。移植试验显示,与股骨、胫骨或骨盆骨髓相比,下颌骨肺泡骨髓的移植率明显增加,这可能是由于肺泡造血干细胞的比例较高。此外,al-BM 中的造血祖细胞(c-Kit+ Sca-1+ Lin-)显示出更强的静止性和更低的凋亡率,表明其具有更优越的维持和存活特性。我们还观察到 al-BM 中间充质基质细胞和骨骼干细胞的富集,这表明 al-BM 的微环境更具支持性。这些研究结果表明,al-BM提供了一种独特的微环境,有利于提高造血干细胞的频率,为特定部位造血提供了新的见解。
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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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