System for Focal, Closed-System Central Nervous System Injury.

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Jove-Journal of Visualized Experiments Pub Date : 2024-11-29 DOI:10.3791/66948
Amy N Stahl, Elisabeth Artis, Purnima Ghose, Tonia S Rex
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引用次数: 0

Abstract

The prevalence of closed-system central nervous system (CNS) injuries underscores the need for an enhanced understanding of these traumas to improve protective and therapeutic interventions. Crucial to this research are animal models that replicate closed-system CNS injuries. In this context, a custom overpressure air system was engineered to reproduce a range of closed-system CNS injuries in murine models, including ocular, brain, and spinal cord trauma. To date, the system has been used to administer eye-, head-, or spine-directed overpressure air to model anteroposterior pole injury in the eye, indirect traumatic optic neuropathy (ITON), focal traumatic brain injury, and spinal cord injury. This paper provides a detailed protocol outlining the system's design and operation and shares representative results demonstrating its effectiveness. The robust framework presented here provides a strong foundation for ongoing research in CNS trauma. By leveraging the system's flexible attributes, investigators can modify and carefully control the location, severity, and timing of injuries. This allows for comprehensive comparisons of molecular mechanisms and therapeutic efficacy across multiple closed-system CNS injuries.

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中枢神经系统局灶性闭合损伤系统
闭合系统中枢神经系统(CNS)损伤的普遍性突出表明,需要加强对这些创伤的了解,以改进保护和治疗干预措施。对这项研究至关重要的是能复制封闭系统中枢神经系统损伤的动物模型。在这种情况下,我们设计了一种定制过压空气系统,用于在小鼠模型中再现一系列闭合系统中枢神经系统损伤,包括眼、脑和脊髓创伤。迄今为止,该系统已被用于向眼睛、头部或脊柱输送过压空气,以模拟眼球前极损伤、间接创伤性视神经病变(ITON)、局灶性脑外伤和脊髓损伤。本文提供了一份详细的协议,概述了该系统的设计和操作,并分享了证明其有效性的代表性结果。本文介绍的强大框架为中枢神经系统创伤的持续研究奠定了坚实的基础。利用该系统的灵活属性,研究人员可以修改并仔细控制损伤的位置、严重程度和时间。这样就可以对多种封闭系统中枢神经系统损伤的分子机制和治疗效果进行综合比较。
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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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