Functional characteristics and mechanisms of microbial community succession and assembly in a long-term moving bed biofilm reactor treating real municipal wastewater.

Abstract

Moving bed biofilm reactor (MBBR) technology with diverse merits is efficient in treating various waste streams whereas their microbial functional properties and ecology still need in-depth investigation, especially in real wastewater treatment systems. Herein, a well-controlled MBBR treating municipal wastewater was established to investigate the long-term system performance and the underlying principles of community succession and assembly. The system successfully achieved ammonium, TN, and chemical oxygen demand (COD) removal of 96.7 ± 2.2%, 75.2 ± 3.6%, and 90.3 ± 3.8%, respectively, under simplified operation and low energy consumption. The effluent TN concentrations achieved 6.2 ± 1.6 mg-N/L despite the influent fluctuations. Diverse functional denitrifiers, such as Denitratisoma, Thermomonas, and Flavobacterium, and the anammox bacteria Candidatus Brocadia successfully enriched in anoxic chamber biofilms. The nitrifiers Nitrosomonas (∼0.73%) and Nitrospira (∼14.0%) exhibited appreciable nitrification capacity in specialized aerobic chambers. Ecological null model and network analysis revealed that microbial community assembly was mainly regulated by niche-based deterministic processes and air diffusion in the aerobic chamber resulted in more intense and complex bacterial interactions. Environmental filters including influent substrate and operating conditions (e.g., reactor configuration, DO, and temperature) greatly shaped the microbial community structure and affected carbon and nitrogen metabolism. The positive ecological roles of influent microflora and functional redundancy in biofilm communities were believed to facilitate functional stability. The anammox process coupled with partial denitrification in a specialized chamber demonstrated positive application implications. These findings provided valuable perspectives in deciphering the microbiological and ecological mechanisms, functional properties, and application potentials of MBBR.