Impact of chemical oxygen demand to nitrogen ratio on ANAMMOX bacterial growth in an up-flow anaerobic sludge blanket reactor.

IF 2.5 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Water Science and Technology Pub Date : 2024-12-01 Epub Date: 2024-11-15 DOI:10.2166/wst.2024.376
Sandile S Msimango, Mahmoud Nasr, Faizal Bux, Sheena Kumari
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Abstract

While several studies have investigated the effect of varying carbon-to-nitrogen (C/N) ratios on the ANAMMOX performance, there is still a research gap in illustrating the shift in 16S rRNA gene copy number and functional microbial population during operation. Hence, this study focuses on utilizing a reference gene and target functional genes to demonstrate the synergetic interaction between ANAMMOX, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB), using an up-flow anaerobic sludge blanket (UASB) under different C/N conditions. It was demonstrated that elevating the C/N ratio from 1.0 to 2.0 reduced the COD and NH4+-N removal efficiencies from 80.12 to 48.62% and from 88.99 to 72.59%, respectively. Based on the qPCR evaluation, at the C/N ratio of 1.5, the abundance of ANAMMOX, AOB, Nitrobacter, and Nitrospira was 2.52 × 106, 82, 5.39 × 103, and 12.98 × 103 copies/μL, respectively. However, with the further increase of C/N ratio to 2.0, their abundance was reduced to 1.09 × 106, 46, 0.98 × 103, and 3.47 × 103 copies/μL, respectively. The expression of hzo gene encoding for hydrazine dehydrogenase was 169-folds at C/N = 1 and almost inhibited at C/N = 2. The results of microbial population structure using 16S rRNA reverse transcriptase (RT)-qPCR technique depicted a competition between ANAMMOX and heterotrophic bacteria for the available substrate at higher C/N ratios.

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化学需氧量与氮比率对上流式厌氧污泥毯反应器中 ANAMMOX 细菌生长的影响。
虽然已有多项研究调查了不同碳氮比(C/N)对 ANAMMOX 性能的影响,但在说明运行过程中 16S rRNA 基因拷贝数和功能微生物种群的变化方面仍存在研究空白。因此,本研究利用参考基因和目标功能基因,重点展示了在不同C/N条件下,使用上流式厌氧污泥毯(UASB)的ANAMMOX、氨氧化细菌(AOB)和亚硝酸盐氧化细菌(NOB)之间的协同作用。结果表明,将 C/N 比从 1.0 提高到 2.0,COD 和 NH4+-N 的去除率分别从 80.12% 和 88.99% 下降到 48.62% 和 72.59%。根据 qPCR 评估,当 C/N 比为 1.5 时,ANAMMOX、AOB、硝化细菌和硝化螺菌的丰度分别为 2.52 × 106、82、5.39 × 103 和 12.98 × 103 个拷贝/μL。然而,当 C/N 比进一步提高到 2.0 时,它们的丰度分别降低到 1.09 × 106、46、0.98 × 103 和 3.47 × 103 个拷贝/μL。编码肼脱氢酶的 hzo 基因的表达量在 C/N = 1 时增加了 169 倍,在 C/N = 2 时几乎被抑制。利用 16S rRNA 逆转录酶(RT)-qPCR 技术研究微生物种群结构的结果表明,在较高的 C/N 比值下,ANAMMOX 与异养菌之间会对可用底物展开竞争。
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来源期刊
Water Science and Technology
Water Science and Technology 环境科学-工程:环境
CiteScore
4.90
自引率
3.70%
发文量
366
审稿时长
4.4 months
期刊介绍: Water Science and Technology publishes peer-reviewed papers on all aspects of the science and technology of water and wastewater. Papers are selected by a rigorous peer review procedure with the aim of rapid and wide dissemination of research results, development and application of new techniques, and related managerial and policy issues. Scientists, engineers, consultants, managers and policy-makers will find this journal essential as a permanent record of progress of research activities and their practical applications.
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