Coumarin-based fluorescent probes for the detection of copper (II) and imaging in mice of Wilson’s disease

IF 4.7 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioorganic Chemistry Pub Date : 2025-01-01 Epub Date: 2024-12-11 DOI:10.1016/j.bioorg.2024.108051

Chunpo Ge, Feng Pei, Xiaoyu Wang, Pengcheng Zhang, Huilin Li, Zhipeng Sai, Yan Yang, Kaiwen Chang, Tianjun Ni, Zhijun Yang

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Abstract

Copper is an essential redox-active metal nutrient, with tightly regulated homeostasis. Copper homeostasis is closely linked to various diseases, making it a critical area of study. Fluorescent probes serve as powerful tools for investigating these conditions. In this study, a fluorescent probe, Con-Cu400 for detecting Cu²⁺ was developed. Con-Cu400 exhibited a strong “turn-on” fluorescence signal in response to Cu²⁺, demonstrating high selectivity and sensitivity. Con-Cu400 also exhibited good biocompatibility and was successfully employed for fluorescence sensing of Cu²⁺ in both cellular and mouse models under conditions of normal, copper-depleted, and copper-overloaded. This probe effectively detected elevated Cu²⁺ levels in the livers of mice with Wilson’s disease. This work may contribute to the broader application of fluorescent probes in studying Cu²⁺ dysregulation-related diseases in cellular and animal models.

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基于香豆素的荧光探针用于检测铜 (II) 和威尔逊氏病小鼠的成像。

铜是一种必需的氧化活性金属营养素，具有严格的体内平衡调节。铜稳态与多种疾病密切相关，是一个重要的研究领域。荧光探针是研究这些条件的有力工具。本研究研制了一种用于检测Cu2+的荧光探针Con-Cu400。Con-Cu400对Cu2+表现出强烈的“开启”荧光信号，具有较高的选择性和灵敏度。Con-Cu400还表现出良好的生物相容性，在正常、缺铜和铜超载的细胞和小鼠模型中成功地用于Cu2+的荧光传感。这种探针有效地检测到了威尔森氏病小鼠肝脏中Cu2+水平的升高。这项工作可能有助于荧光探针在细胞和动物模型中研究Cu2+失调相关疾病的更广泛应用。

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.