Rapid diagnostic testing method to detect ROB β-lactamase gene in Neisseria meningitidis.

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Molecular and Cellular Probes Pub Date : 2024-12-19 DOI:10.1016/j.mcp.2024.102000
Andrew Peifer, Anna Kidney, Geetha Nattanmai, Kate Wahl, Sherly Jose, Elizabeth Owuor, Linnell Randall, Erin Klingbeil, Kimberlee A Musser, Kara Mitchell
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引用次数: 0

Abstract

blaROB-1 is the only widely found β-lactamase in Neisseria meningitidis, and its presence is on the rise. To enhance our bacterial meningitis testing procedure, we clinically validated a real-time PCR assay to rapidly detect the blaROB gene and predict drug resistance in Neisseria meningitidis. A screen of 101 clinical isolates and 37 clinical specimens of blood and cerebrospinal fluid received between January 2018 and June 2024 found 8 isolates and 2 cerebrospinal fluid specimens that were positive for blaROB.

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检测脑膜炎奈瑟菌 ROB β-内酰胺酶基因的快速诊断测试法
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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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