Protocol for the development of a bioluminescent AML-PDX mouse model for the evaluation of CAR T cell therapy.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-12 DOI:10.1016/j.xpro.2024.103522
Mireia Mayoral Safont, Calum Leitch, Mihaela Popa, May Eriksen Gjerstad, Benjamin Caulier, Else Marit Inderberg, Sébastien Wälchli, Pascal Gelebart, Emmet Mc Cormack
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Abstract

Patient-derived xenograft (PDX) models of acute myeloid leukemia (AML-PDX) offer advantages over cell line models by capturing the complexity and heterogeneity of patient-derived samples. Here, we present a protocol for developing a bioluminescent AML-PDX model in mice to evaluate chimeric antigen receptor (CAR) T cell therapy. We describe steps for transducing, engrafting, expanding, and enriching AML-PDX cells. We then detail procedures for in vitro and in vivo validation of the AML-PDX model for the evaluation of CAR T cell immunotherapy. For complete details on the use and execution of this protocol, please refer to Caulier et al.1.

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开发用于评估 CAR T 细胞疗法的生物发光 AML-PDX 小鼠模型的方案。
急性髓系白血病(AML-PDX)患者源性异种移植(PDX)模型通过捕获患者源性样本的复杂性和异质性,比细胞系模型具有优势。在这里,我们提出了一种开发小鼠生物发光AML-PDX模型的方案,以评估嵌合抗原受体(CAR) T细胞治疗。我们描述了转导、移植、扩增和富集AML-PDX细胞的步骤。然后,我们详细介绍了用于评估CAR - T细胞免疫疗法的AML-PDX模型的体外和体内验证程序。有关本协议使用和执行的完整细节,请参见Caulier等人1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
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