Ethylene response transcription factor 5 (ERF5) enhances defense against tobacco curly shoot virus and associated betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana.

Virology Pub Date : 2024-11-29 DOI:10.1016/j.virol.2024.110309

Meisheng Zhao, Liping Zhang, Hussein Ghanem, Gentu Wu, Mingjun Li, Ling Qing

{"title":"Ethylene response transcription factor 5 (ERF5) enhances defense against tobacco curly shoot virus and associated betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana.","authors":"Meisheng Zhao, Liping Zhang, Hussein Ghanem, Gentu Wu, Mingjun Li, Ling Qing","doi":"10.1016/j.virol.2024.110309","DOIUrl":null,"url":null,"abstract":"<p><p>Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. Nicotiana benthamiana is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 NbERFs that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and Niben101Scf00163g22002 (NbERF5) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing NbERF5 enhances TbCSV/TbCSB infection, and overexpression of NbERF5 inhibits TbCSV/TbCSB infection. Importantly, NbERF5 positively regulates the expression of the pathogenesis-related (PR) genes, NbPR1a and NbNPR1. Our findings suggest that NbERF5 enhances TbCSV/TbCSB resistance by activating the PR genes, indicating that NbERF5 is a potential antiviral gene.</p>","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110309"},"PeriodicalIF":0.0000,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.virol.2024.110309","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. Nicotiana benthamiana is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 NbERFs that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and Niben101Scf00163g22002 (NbERF5) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing NbERF5 enhances TbCSV/TbCSB infection, and overexpression of NbERF5 inhibits TbCSV/TbCSB infection. Importantly, NbERF5 positively regulates the expression of the pathogenesis-related (PR) genes, NbPR1a and NbNPR1. Our findings suggest that NbERF5 enhances TbCSV/TbCSB resistance by activating the PR genes, indicating that NbERF5 is a potential antiviral gene.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

乙烯反应转录因子5（ERF5）可增强烟草卷曲芽病毒及相关β卫星（TbCSV/TbCSB）在烟草中的防御能力。

begomvirus / betassatellite疫病复合体严重威胁全球作物生产。鉴定潜在的植物抗病毒基因对疾病控制至关重要。本烟对病毒敏感，含有266个乙烯应答转录因子（ERFs）。本研究发现29个nberf在烟草卷枝病毒及其相关的betasatsatellite （TbCSV/TbCSB）感染中差异上调，其中ERF5最为常见。9个NbERF5簇在系统发育上和Niben101Scf00163g22002 （NbERF5）对外源性ACC处理有显著反应。进一步分析证实了NbERF5的核定位和转录激活活性。蛋白相互作用实验表明，NbERF5无自相互作用，不与TbCSB βC1蛋白相互作用。沉默NbERF5可增强TbCSV/TbCSB感染，过表达NbERF5可抑制TbCSV/TbCSB感染。重要的是，NbERF5正调控致病相关（PR）基因NbPR1a和NbNPR1的表达。我们的研究结果表明，NbERF5通过激活PR基因增强TbCSV/TbCSB抗性，表明NbERF5是一个潜在的抗病毒基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Virology

自引率

0.00%

发文量