Structural Analysis of the LZTFL1 Protein by Principal Component Analysis (PCA-seq)

Abstract

Objective: The single-nucleotide mutation rs17713054G>A in the promoter region of LZTFL1 (leucine zipper transcription factor like 1) gene is a factor in the severe course of coronavirus infection COVID-19. Structure of coded protein is not studied well still. Methods: We used the principal component analysis (PCA-seq) for computer statistical analysis of protein LZTFL1 aminoacid sequence. Results and Discussion: It is revealed that the presence of a high correlation between the first principal component of the translated amino acid sequence and eleven amino acid indices of the AA-index database, characterizing the physicochemical and biochemical properties of the protein. The indices BEGF750102, CHOP780209, PALJ810110, GEIM800107, QIAN880121, LEVM780102, PRAM900103 are associated with β-folding parameters. The LZTFL1 protein is part of the Bardet-Biedl Syndrome (BBS) protein complexes that regulate intracellular transport in the ciliated epithelium of the lungs. Conclusions: It is assumed that the presence of β-sheet elements in the structure of the LZTFL1 protein plays an important role in ACE2 receptor-mediated endocytosis, stimulating the rate of angiotensin-converting enzyme 2 recycling and accelerating the delivery of adherented coronavirus SARS-CoV-2 virions into the cell followed by the initiation of severe acute respiratory syndrome COVID-19.