Proteomic and spectral analysis reveals the role of extracellular polymeric substances in mercury biosorption by activated sludge under high-altitude conditions.

IF 7.7 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Environmental Research Pub Date : 2024-12-14 DOI:10.1016/j.envres.2024.120613
Lei Gao, Yue Chen, Shuping Li, Zhonglian Yang, Yongze Lu, Guangcan Zhu
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Abstract

In high-altitude regions, elevated mercury (Hg) levels in wastewater treatment plants (WWTPs) influent raise concerns about treatment efficiency and environmental impact. This study investigated the Hg biosorption capacity of activated sludge under high-altitude conditions, focusing on the binding mechanisms between EPS and Hg, and variations in EPS secretion. Low pressure, oxygen, and temperature at high altitudes increase EPS secretion, enhancing Hg biosorption. EPS provides numerous binding sites for Hg, forming nonfluorescent complexes with tryptophan-like and aromatic proteins, while hydrocarbon and oxygen-containing groups limit Hg entry into microbial cells. Proteomic analysis revealed the upregulation of transporters, stress-resistance, and binding proteins, along with those involved in carbon and amino acid metabolism, which enhance microbial resilience and EPS production, leading to increased Hg biosorption. These insights reveal adaptive mechanisms that optimize pollutant removal in high-altitude environments.

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蛋白质组和光谱分析揭示了细胞外高分子物质在高海拔条件下活性污泥对汞的生物吸附中的作用。
在高海拔地区,污水处理厂(WWTPs)进水中汞含量的升高引起了人们对处理效率和环境影响的关注。本研究调查了高海拔条件下活性污泥对汞的生物吸附能力,重点研究了 EPS 与汞的结合机制以及 EPS 分泌的变化。高海拔地区的低压、低氧和低温会增加 EPS 的分泌,从而增强对汞的生物吸附能力。EPS 为汞提供了大量结合位点,与色氨酸类和芳香族蛋白形成无荧光复合物,而碳氢化合物和含氧基团则限制了汞进入微生物细胞。蛋白质组分析表明,转运蛋白、抗应激蛋白、结合蛋白以及参与碳和氨基酸代谢的蛋白上调,这增强了微生物的恢复能力和 EPS 的产生,从而提高了汞的生物吸附能力。这些见解揭示了在高海拔环境中优化污染物清除的适应机制。
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来源期刊
Environmental Research
Environmental Research 环境科学-公共卫生、环境卫生与职业卫生
CiteScore
12.60
自引率
8.40%
发文量
2480
审稿时长
4.7 months
期刊介绍: The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.
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