Effect of pH on antitumor activity of Chinese cobra (Naja atra) cytotoxin-XII.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Cytotechnology Pub Date : 2025-02-01 Epub Date: 2024-12-13 DOI:10.1007/s10616-024-00681-8
Xiancai Su, Jiayi Zhou, Mingyuan Zhang, Xiaoping Kang, Dongli Lu, Yanling Chen, Qing Lin, Cailing Yan, Yunlu Xu
{"title":"Effect of pH on antitumor activity of Chinese cobra (Naja atra) cytotoxin-XII.","authors":"Xiancai Su, Jiayi Zhou, Mingyuan Zhang, Xiaoping Kang, Dongli Lu, Yanling Chen, Qing Lin, Cailing Yan, Yunlu Xu","doi":"10.1007/s10616-024-00681-8","DOIUrl":null,"url":null,"abstract":"<p><p>Cytotoxins (CTXs), proteins found in cobra venom, selectively inhibit tumor cell proliferation. Herein, we selected CTX-XII because of its potent antitumor activity to investigate the effect of solution pH on its response. MTT assay results showed significantly higher inhibition rates for CTX-XII at pH 5.72 (75.79 ± 3.48%) than that at pH 7.32 (50.75 ± 3.8%). Flow cytometry demonstrated that apoptosis rates in B16F10 cells induced by CTX-XII were also higher at pH 5.72 (44.92 ± 7.94%) and 4.12 (42.87 ± 1.89%) than at pH 7.32 (23.5 ± 4.02%). Confocal laser scanning microscopy images showed that red fluorescence, representing CTX-XII concentration, was more intense around tumor cells at pH 5.72, with higher levels in the cytoplasm, than at pH 7.32. In the murine melanoma model, tumor weight in the pH 5.72 CTX-XII group (0.45 ± 0.19 g) was significantly lower than that in the pH 7.32 CTX-XII group (0.84 ± 0.42 g). These results indicate that pH has a strong influence on the antitumor activity of CTX-XII, likely due to pH-dependent ionization changes in CTX-XII that increase its affinity for and penetration into tumor cell membranes. This study provides new insights into the antitumor effects of CTXs and factors influencing their activity.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-024-00681-8.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 1","pages":"21"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645386/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10616-024-00681-8","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/13 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Cytotoxins (CTXs), proteins found in cobra venom, selectively inhibit tumor cell proliferation. Herein, we selected CTX-XII because of its potent antitumor activity to investigate the effect of solution pH on its response. MTT assay results showed significantly higher inhibition rates for CTX-XII at pH 5.72 (75.79 ± 3.48%) than that at pH 7.32 (50.75 ± 3.8%). Flow cytometry demonstrated that apoptosis rates in B16F10 cells induced by CTX-XII were also higher at pH 5.72 (44.92 ± 7.94%) and 4.12 (42.87 ± 1.89%) than at pH 7.32 (23.5 ± 4.02%). Confocal laser scanning microscopy images showed that red fluorescence, representing CTX-XII concentration, was more intense around tumor cells at pH 5.72, with higher levels in the cytoplasm, than at pH 7.32. In the murine melanoma model, tumor weight in the pH 5.72 CTX-XII group (0.45 ± 0.19 g) was significantly lower than that in the pH 7.32 CTX-XII group (0.84 ± 0.42 g). These results indicate that pH has a strong influence on the antitumor activity of CTX-XII, likely due to pH-dependent ionization changes in CTX-XII that increase its affinity for and penetration into tumor cell membranes. This study provides new insights into the antitumor effects of CTXs and factors influencing their activity.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-024-00681-8.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
pH 值对眼镜蛇细胞毒素-XII 抗肿瘤活性的影响
细胞毒素(CTXs)是眼镜蛇毒液中的蛋白质,可选择性地抑制肿瘤细胞的增殖。由于 CTX-XII 具有很强的抗肿瘤活性,我们选择了它来研究溶液 pH 值对其反应的影响。MTT 检测结果显示,CTX-XII 在 pH 值为 5.72 时的抑制率(75.79 ± 3.48%)明显高于 pH 值为 7.32 时的抑制率(50.75 ± 3.8%)。流式细胞仪显示,CTX-XII 诱导的 B16F10 细胞凋亡率在 pH 值为 5.72(44.92 ± 7.94%)和 4.12(42.87 ± 1.89%)时也高于 pH 值为 7.32(23.5 ± 4.02%)时。共焦激光扫描显微镜图像显示,pH 值为 5.72 时肿瘤细胞周围的红色荧光(代表 CTX-XII 浓度)比 pH 值为 7.32 时更强,细胞质中的含量更高。在小鼠黑色素瘤模型中,pH 值为 5.72 的 CTX-XII 组肿瘤重量(0.45 ± 0.19 克)明显低于 pH 值为 7.32 的 CTX-XII 组(0.84 ± 0.42 克)。这些结果表明,pH值对CTX-XII的抗肿瘤活性有很大影响,这可能是由于CTX-XII的电离变化依赖于pH值,从而增加了其对肿瘤细胞膜的亲和力和穿透力。这项研究为CTX的抗肿瘤作用以及影响其活性的因素提供了新的见解:在线版本包含补充材料,可查阅 10.1007/s10616-024-00681-8。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
期刊最新文献
Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture. IBS008738, a TAZ activator, facilitates muscle repair and inhibits muscle injury in a mouse model of sport-induced injury. Yinjia pills inhibits the malignant biological behavior of HeLa cells through PKM2-medicated inhibition of JAK/STAT3 pathway. Functional improvements in β-lactoglobulin by conjugation with high methoxy pectin by the Maillard reaction. Etomidate suppresses proliferation, migration, invasion, and glycolysis in esophageal cancer cells via PI3K/AKT pathway inhibition.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1