The impact of multiplex nested gastrointestinal PCR panel in children with gastroenteridis requiring pediatric infectious disease consultation.

IF 3 3区医学 Q1 PEDIATRICS European Journal of Pediatrics Pub Date : 2024-12-16 DOI:10.1007/s00431-024-05918-4

Deniz Ergün, Pelin Kaçar, Hıncal Özbakır, Mustafa Gülderen, Miray Yılmaz Çelebi, Ege Gürbüz, Gizem Güner Özenen, Arife Özer, Aybüke Akaslan Kara, Fahri Yüce Ayhan, Çigdem Ömür Ecevit, Özlem Bekem, Süleyman Nuri Bayram, İlker Devrim

{"title":"The impact of multiplex nested gastrointestinal PCR panel in children with gastroenteridis requiring pediatric infectious disease consultation.","authors":"Deniz Ergün, Pelin Kaçar, Hıncal Özbakır, Mustafa Gülderen, Miray Yılmaz Çelebi, Ege Gürbüz, Gizem Güner Özenen, Arife Özer, Aybüke Akaslan Kara, Fahri Yüce Ayhan, Çigdem Ömür Ecevit, Özlem Bekem, Süleyman Nuri Bayram, İlker Devrim","doi":"10.1007/s00431-024-05918-4","DOIUrl":null,"url":null,"abstract":"Infectious gastroenteritis is an important cause of morbidity and mortality, especially in nations with middle and low incomes. In a pediatric gastroenteritis patient, the aim was to determine the therapeutic impact of using the Gastrointestinal Panel in our clinical practice. A single-center retrospective study was designed to evaluate children who were admitted to the hospital for gastroenteritis and had a gastrointestinal panel between August 2021 and January 2024. 103 patients who had gastrointestinal (GI) panel results were included in the study. The GI Panel positivity rate among 103 patients was 55.3% (n = 57). Bacterial agents were positive in 25 patients (43.8%), viral agents were found in 11 patients (19.2%), and polymicrobial agents were found positive in 21 patients (36.8%). Escherichia coli (9.1%) was the most common bacterial pathogen, and viral pathogens most frequently included Rotavirus (11.6%) and Norovirus (11.6%). When the effect of the GI Panel test on treatment was determined, the treatment of 51 (49.5%) patients was changed according to GI Panel.Conclusions: In our study, the treatment regimen of many patients was adjusted based on the GI panel results in patients presenting with gastroenteritis. GI panel had an important impact on the patients care and optimization according to the principles of antimicrobial treatment. GI panel tests had several advantages such as speed and diagnostic accuracy as good as stool culture.What is known: • Infectious gastroenteritis is a major cause of morbidity and mortality, especially in middle- and low-income countries. • BioFire® FilmArray, Gastrointestinal Panel (BioFire- Biomerieux, France) is a rapid and simple technique that uses nested multiplex PCR to detect gastrointestinal pathogens rapidly.What is new: • In this study, the GI Panel test was found to have a cumulative impact on 49.5% (n=51) of the clinician's treatment modalities. • With its speed and diagnostic sensitivity, the GI Panel may provide clinicians with an important tool in the fight against antibiotic resistance by decreasing antimicrobial drug treatment at the children with gastroenteritis.","PeriodicalId":11997,"journal":{"name":"European Journal of Pediatrics","volume":"184 1","pages":"85"},"PeriodicalIF":3.0000,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Pediatrics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00431-024-05918-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PEDIATRICS","Score":null,"Total":0}

引用次数: 0

Abstract

Infectious gastroenteritis is an important cause of morbidity and mortality, especially in nations with middle and low incomes. In a pediatric gastroenteritis patient, the aim was to determine the therapeutic impact of using the Gastrointestinal Panel in our clinical practice. A single-center retrospective study was designed to evaluate children who were admitted to the hospital for gastroenteritis and had a gastrointestinal panel between August 2021 and January 2024. 103 patients who had gastrointestinal (GI) panel results were included in the study. The GI Panel positivity rate among 103 patients was 55.3% (n = 57). Bacterial agents were positive in 25 patients (43.8%), viral agents were found in 11 patients (19.2%), and polymicrobial agents were found positive in 21 patients (36.8%). Escherichia coli (9.1%) was the most common bacterial pathogen, and viral pathogens most frequently included Rotavirus (11.6%) and Norovirus (11.6%). When the effect of the GI Panel test on treatment was determined, the treatment of 51 (49.5%) patients was changed according to GI Panel.

Conclusions: In our study, the treatment regimen of many patients was adjusted based on the GI panel results in patients presenting with gastroenteritis. GI panel had an important impact on the patients care and optimization according to the principles of antimicrobial treatment. GI panel tests had several advantages such as speed and diagnostic accuracy as good as stool culture.

What is known: • Infectious gastroenteritis is a major cause of morbidity and mortality, especially in middle- and low-income countries. • BioFire® FilmArray, Gastrointestinal Panel (BioFire- Biomerieux, France) is a rapid and simple technique that uses nested multiplex PCR to detect gastrointestinal pathogens rapidly.

What is new: • In this study, the GI Panel test was found to have a cumulative impact on 49.5% (n=51) of the clinician's treatment modalities. • With its speed and diagnostic sensitivity, the GI Panel may provide clinicians with an important tool in the fight against antibiotic resistance by decreasing antimicrobial drug treatment at the children with gastroenteritis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

多重巢式胃肠道 PCR 小组对需要儿科传染病会诊的肠胃炎患儿的影响。

传染性肠胃炎是发病和死亡的重要原因，尤其是在中低收入国家。在小儿肠胃炎患者中，我们的目的是确定在临床实践中使用胃肠镜的治疗效果。我们设计了一项单中心回顾性研究，对 2021 年 8 月至 2024 年 1 月期间因肠胃炎入院并进行胃肠道检查的儿童进行评估。研究共纳入了 103 名有胃肠道检查结果的患者。在 103 名患者中，胃肠样本阳性率为 55.3%（n = 57）。有 25 名患者（43.8%）发现细菌病原体阳性，11 名患者（19.2%）发现病毒病原体阳性，21 名患者（36.8%）发现多微生物病原体阳性。大肠杆菌（9.1%）是最常见的细菌病原体，病毒病原体最常见的是轮状病毒（11.6%）和诺如病毒（11.6%）。在确定 GI Panel 检测对治疗的影响时，51 名（49.5%）患者的治疗方案根据 GI Panel 而改变：结论：在我们的研究中，许多患者的治疗方案都是根据胃肠炎患者的消化道检查结果进行调整的。根据抗菌治疗原则，消化道检查对患者的护理和优化治疗方案有重要影响。消化道全套检测具有多项优势，如速度快、诊断准确性不亚于粪便培养：- 传染性肠胃炎是发病和死亡的主要原因，尤其是在中等收入和低收入国家。- BioFire® FilmArray 胃肠道检测试剂盒（BioFire- Biomerieux，法国）是一种快速简便的技术，利用巢式多重 PCR 技术快速检测胃肠道病原体： - 新发现：在这项研究中，胃肠道样本检测对临床医生 49.5%（n=51）的治疗方式产生了累积影响。- 凭借其速度和诊断灵敏度，胃肠道样本检测仪可减少肠胃炎患儿的抗菌药物治疗，从而为临床医生提供对抗抗生素耐药性的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

European Journal of Pediatrics 医学-小儿科

CiteScore

5.90

自引率

2.80%

发文量

367

审稿时长

3-6 weeks

期刊介绍： The European Journal of Pediatrics (EJPE) is a leading peer-reviewed medical journal which covers the entire field of pediatrics. The editors encourage authors to submit original articles, reviews, short communications, and correspondence on all relevant themes and topics. EJPE is particularly committed to the publication of articles on important new clinical research that will have an immediate impact on clinical pediatric practice. The editorial office very much welcomes ideas for publications, whether individual articles or article series, that fit this goal and is always willing to address inquiries from authors regarding potential submissions. Invited review articles on clinical pediatrics that provide comprehensive coverage of a subject of importance are also regularly commissioned. The short publication time reflects both the commitment of the editors and publishers and their passion for new developments in the field of pediatrics. EJPE is active on social media (@EurJPediatrics) and we invite you to participate. EJPE is the official journal of the European Academy of Paediatrics (EAP) and publishes guidelines and statements in cooperation with the EAP.