A chromosome-level genome of Lobelia seguinii provides insights into the evolution of Campanulaceae and the lobeline biosynthesis.

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genomics Pub Date : 2024-12-13 DOI:10.1016/j.ygeno.2024.110979

Na Zhang, Puguang Zhao, Wenda Zhang, Huiying Wang, Kaixuan Wang, Xiangyu Wang, Zhanjing Zhang, Ninghua Tan, Lingyun Chen

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Abstract

Lobelia seguinii is a plant with great ecological and medicinal value and belongs to Campanulaceae. Lobelia contains lobeline, a well-known compound used to treat respiratory diseases. Nevertheless, lobeline biosynthesis needs further exploration. Moreover, whole-genome duplication (WGD) and karyotype evolution within Campanulaceae still need to be better understood. In this study, we obtained a chromosome-level genome of L. seguinii with a size of 1.4 Gb and 38,253 protein-coding genes. Analyses revealed two WGDs within Campanulaceae, one at the most recent common ancestor (MRCA) of Campanula and Adenophora, and another at the MRCA of Lobelioideae. Analyses further revealed that the karyotype of Platycodon grandiflorus represents the ancient type within Asterales. We proposed eight enzymes involved in the lobeline biosynthesis pathway of L. seguinii. Molecular cloning and heterologous expression of phenylalanine ammonia-lyase (PAL), a candidate enzyme involved in the first step of lobeline biosynthesis, verified its function to catalyze the deamination of phenylalanine to cinnamic acid. This study sheds light on the evolution of Campanulaceae and lobeline biosynthesis.

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Lobelia seguinii 是一种具有重要生态和药用价值的植物，属于凌霄花科。龙须菜含有龙须菜碱，这是一种用于治疗呼吸道疾病的著名化合物。然而，龙脑香碱的生物合成还需要进一步探索。此外，人们还需要更好地了解凌霄花科植物的全基因组复制（WGD）和核型演化。在这项研究中，我们获得了一个染色体组水平的 L. seguinii 基因组，大小为 1.4 Gb，包含 38,253 个蛋白编码基因。分析表明，在凌霄花科（Campanulaceae）中存在两个WGD，一个位于凌霄花科和Adenophora科的最近共同祖先（MRCA）处，另一个位于Lobelioideae科的最近共同祖先（MRCA）处。分析进一步表明，桔梗的核型代表了菊科植物中的古老类型。我们提出了参与L. seguinii小叶碱生物合成途径的八种酶。苯丙氨酸氨解酶（PAL）是参与小叶碱生物合成第一步的候选酶，其分子克隆和异源表达验证了其催化苯丙氨酸脱氨为肉桂酸的功能。这项研究揭示了钟乳石科植物和龙脑香脂生物合成的进化过程。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.