Higher Body Mass Index is Associated with Increased Oxidative Stress in Patients of Type 2 Diabetes Mellitus.

Indian Journal of Endocrinology and Metabolism Pub Date : 2024-09-01 Epub Date: 2024-09-04 DOI:10.4103/ijem.ijem_474_23

Edelbert A Almeida, Mohit Mehndiratta, Sri V Madhu, Rajarshi Kar

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Abstract

Introduction: Type 2 diabetes mellitus (T2DM) is closely associated with the obesity; however, certain proportion of T2DM patients is non-obese or lean (BMI < 18.5 kg/m²). Obesity has long been associated with oxidative stress; however, there are no studies available documenting levels of oxidative stress in the lean patients of T2DM. Therefore, this study was done to compare the levels of makers of oxidative stress (TL, mtDNA, TAS) and their regulators (mRNA expression of TERT, Nrf2 and Nqo1) in lean and obese patients of T2DM.

Methods: 60 newly diagnosed patients (treatment naïve) of T2DM were recruited and divided into lean (BMI < 18.5 kg/m²) and obese (BMI > 25 kg/m²) groups. Relative telomere length (T/S) and mtDNA content were estimated via real-time PCR. Serum total antioxidant status (TAS) was measured using a commercially available kit. mRNA expression of TERT, Nrf2 and Nqo1 was measured by real-time PCR.

Results: Mean T/S and mtDNA content were lower in the obese group compared to the lean group (P = 0.16 and P = 0.06, respectively). Mean serum TAS levels were higher in obese group compared to the lean group (P = 0.001). mRNA expression of TERT and Nrf2 was increased in obese group compared to the lean group. mRNA expression of Nqo1 was similar in both the groups.

Conclusion: Obese patients of T2DM are exposed to a greater degree of OS compared to the lean patients of T2DM.

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高体重指数与2型糖尿病患者氧化应激增加有关

2型糖尿病（T2DM）与肥胖密切相关；但也有一定比例的T2DM患者非肥胖或偏瘦（BMI < 18.5 kg/m2）。长期以来，肥胖一直与氧化应激有关；然而，目前尚无研究记录瘦型2型糖尿病患者的氧化应激水平。因此，本研究比较了瘦型和肥胖型T2DM患者氧化应激生成因子（TL、mtDNA、TAS）及其调节因子（TERT、Nrf2、Nqo1 mRNA表达）的水平。方法：招募60例新诊断T2DM患者（治疗naïve），分为瘦组（BMI < 18.5 kg/m2）和肥胖组（BMI > 25 kg/m2）。实时荧光定量PCR测定相对端粒长度（T/S）和mtDNA含量。血清总抗氧化状态（TAS）用市售试剂盒测定。实时荧光定量PCR检测TERT、Nrf2、Nqo1 mRNA表达量。结果：肥胖组平均T/S和mtDNA含量低于瘦组（P = 0.16和P = 0.06）。肥胖组平均血清TAS水平高于瘦肉组（P = 0.001）。肥胖组TERT和Nrf2 mRNA表达量明显高于瘦肉组。两组Nqo1 mRNA表达相似。结论：肥胖T2DM患者比瘦弱T2DM患者暴露于更大程度的OS。

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来源期刊

Indian Journal of Endocrinology and Metabolism Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

2.10

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0.00%

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期刊介绍： The Indian Journal of Endocrinology and Metabolism (IJEM) aims to function as the global face of Indian endocrinology research. It aims to act as a bridge between global and national advances in this field. The journal publishes thought-provoking editorials, comprehensive reviews, cutting-edge original research, focused brief communications and insightful letters to editor. The journal encourages authors to submit articles addressing aspects of science related to Endocrinology and Metabolism in particular Diabetology. Articles related to Clinical and Tropical endocrinology are especially encouraged. Sub-topic based Supplements are published regularly. This allows the journal to highlight issues relevant to Endocrine practitioners working in India as well as other countries. IJEM is free access in the true sense of the word, (it charges neither authors nor readers) and this enhances its global appeal.