Integration of large genetic payloads using prime editing and site-specific integrases

IF 16 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS Nature Protocols Pub Date : 2024-12-15 DOI:10.1038/s41596-024-01094-9

Zongliang Gao, Rasmus O. Bak

引用次数: 0

Abstract

A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.

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利用启动编辑和位点特异性整合酶整合大的遗传有效载荷。

最近的PASTE（通过位点特异性靶向元件可编程添加）协议结合了启动编辑和位点特异性整合酶来整合大的遗传有效载荷。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Protocols 生物-生化研究方法

CiteScore

29.10

自引率

0.70%

发文量

128

审稿时长

4 months

期刊介绍： Nature Protocols focuses on publishing protocols used to address significant biological and biomedical science research questions, including methods grounded in physics and chemistry with practical applications to biological problems. The journal caters to a primary audience of research scientists and, as such, exclusively publishes protocols with research applications. Protocols primarily aimed at influencing patient management and treatment decisions are not featured. The specific techniques covered encompass a wide range, including but not limited to: Biochemistry, Cell biology, Cell culture, Chemical modification, Computational biology, Developmental biology, Epigenomics, Genetic analysis, Genetic modification, Genomics, Imaging, Immunology, Isolation, purification, and separation, Lipidomics, Metabolomics, Microbiology, Model organisms, Nanotechnology, Neuroscience, Nucleic-acid-based molecular biology, Pharmacology, Plant biology, Protein analysis, Proteomics, Spectroscopy, Structural biology, Synthetic chemistry, Tissue culture, Toxicology, and Virology.