{"title":"Proteome Analysis of the Nitric Oxide Donor Effect on Pisum Sativum L. Roots","authors":"A. M. Egorova, I. A. Tarchevsky","doi":"10.1134/S0003683824604694","DOIUrl":null,"url":null,"abstract":"<p>Proteomic analysis of the NO donor–sodium nitroprusside effect on pea seedlings roots revealed a high degree of induction of the platform propeller protein WD40, PR10, ferritin, and the chitinase-like protein. Less induction was observed for ABR17, the endochitinase-like protein, enzymes of phytoalexins synthesis, and proline iminopeptidase, which releases proline from proline-enriched proteins, and UTP-glucose-1-P-uridylyl transferase involved in the synthesis of cellulose and callose. Some of these defense compounds, such as endochitinase, chitinase-like protein, and phenolic phytoalexins, are transported into the rhizosphere and may influence the rhizosphere microbiome. There are examples of the use of NO defense properties in agrotechnology both for treating plants with NO donors and for creating stress-resistant transgenic plants with a high content of NO or compounds induced by it (ferritin, etc.).</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1292 - 1300"},"PeriodicalIF":1.0000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824604694","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Proteomic analysis of the NO donor–sodium nitroprusside effect on pea seedlings roots revealed a high degree of induction of the platform propeller protein WD40, PR10, ferritin, and the chitinase-like protein. Less induction was observed for ABR17, the endochitinase-like protein, enzymes of phytoalexins synthesis, and proline iminopeptidase, which releases proline from proline-enriched proteins, and UTP-glucose-1-P-uridylyl transferase involved in the synthesis of cellulose and callose. Some of these defense compounds, such as endochitinase, chitinase-like protein, and phenolic phytoalexins, are transported into the rhizosphere and may influence the rhizosphere microbiome. There are examples of the use of NO defense properties in agrotechnology both for treating plants with NO donors and for creating stress-resistant transgenic plants with a high content of NO or compounds induced by it (ferritin, etc.).
一氧化氮给体硝普钠对豌豆幼苗根系的蛋白质组学分析显示，平台螺旋桨蛋白WD40、PR10、铁蛋白和几丁质酶样蛋白高度诱导。ABR17、内几丁质酶样蛋白、植物抗毒素合成酶、脯氨酸亚胺肽酶（从富含脯氨酸的蛋白质中释放脯氨酸）和参与纤维素和胼胶合成的utp -葡萄糖-1- p -尿苷基转移酶的诱导作用较弱。其中一些防御化合物，如几丁质内酶、几丁质酶样蛋白和酚类植物抗毒素，被运输到根际，并可能影响根际微生物群。在农业技术中有使用NO防御特性的例子，既用于处理有NO供体的植物，也用于制造具有高含量NO或由其诱导的化合物（铁蛋白等）的抗胁迫转基因植物。
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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