The global attention to the utilization of unmanned aerial vehicle remote sensing drones in crop disease-wide detection has led to the urgent need to find an adapted model for different environmental conditions. Therefore, the current study has focused on spatiotemporal usage of different multispectral cameras in acquiring spectral reflectance models of in-field rice bacterial blight stresses. Where, long short-term memory (LSTM) model was compared with the other models in transfer learning strategy for assessing the blight stress severity. The results revealed that by extracting 30% of the data from the target domain and transferring it to the source domain, the adaptability of the model across different sites was effectively enhanced. Besides, LSTM showed high tuning transfer efficiency that demonstrated optimal predictive performance and the shortest training time in transfer tasks. Its coefficient of the prediction set was 0.82, and its residual prediction deviation has reached 2.26. In practice, LSTM enabled the acquisition of reliable prediction results at a minimal sample collection cost while circumventing feature reduction resulting from inter-domain data alignment. When the transfer ratio reached 20%, the coefficient of determination of the prediction set reached 0.71, and the residual prediction deviation reached 1.79. The novelty of this study came from the transfer learning efficiency in improving the model’s application capabilities across the different sites, environment, and unmanned aerial vehicle in farmland disease detection.