Palladium Nanoparticle-Decorated Copper-Hemin Metal Organic Framework for Enzymatic Electrochemical Detection of Creatinine in Human Urine

Abstract

Creatinine is indeed a crucial biomarker for kidney diseases. In this work, a novel electrochemical biosensor based on a copper-hemin metal organic framework [Cu-hemin metal–organic framework (MOF)] nanoflake decorated with palladium (Pd) (Pd/Cu-hemin MOF) was fabricated and incorporated with creatinine deiminase (CD) on a glassy carbon electrode (GCE) for creatinine detection. The formation of a Pd/Cu-hemin MOF composite was confirmed by X-ray photoelectron spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The formation of the composite as nanoflakes is evident from the scanning electron microscopy image. The transmission electron microscopy image clarifies the decoration of palladium nanoparticles on Cu-hemin MOF surfaces. Thus, the proposed biosensor (Pd/Cu-hemin MOF/CD/GCE) electrochemical performances were studied with cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy. As a result, the Pd/Cu-hemin MOF/CD/GCE-based electrochemical detection of creatinine exhibits a broad linear range from 0 to 130 μM (R² = 0.99), a low limit of detection 0.08 μM, and an excellent sensitivity of 3.2 μA μM^–1 cm^–2. The biosensor also determines creatinine in samples of human urine with a good recovery from 99.4 to 100.8%. Thus, in this study, an electrochemical biosensing platform based on Pd/Cu-hemin MOF/CD/GCE has been designed practically for creatinine.