Alkali burn injury model of meibomian gland dysfunction in mice.

IF 1.9 4区医学 Q2 OPHTHALMOLOGY International journal of ophthalmology Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI:10.18240/ijo.2024.12.02

Yong Li, Ya-Qiong Yang, Yong Lin, Ke Yan, Yu-Fei Lyu, Zhao-Qiang Zhang, Cai-Hong Huang, Jiao-Yue Hu, Zu-Guo Liu

{"title":"Alkali burn injury model of meibomian gland dysfunction in mice.","authors":"Yong Li, Ya-Qiong Yang, Yong Lin, Ke Yan, Yu-Fei Lyu, Zhao-Qiang Zhang, Cai-Hong Huang, Jiao-Yue Hu, Zu-Guo Liu","doi":"10.18240/ijo.2024.12.02","DOIUrl":null,"url":null,"abstract":"Aim: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD).Methods: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model. The other eye was left untreated as a control group. Eyelid margin morphological changes and the meibomian glands (MGs) were observed by slit lamp microscopy on days 5 and 10 post-burn. Hematoxylin-eosin (HE) staining and Oil red O staining were adopted in detecting the changes in MGs morphology and lipid deposition. Real-time polymerase chain reaction, Western blot, immunofluorescence staining and immunohistochemical staining were used to detect interleukin (IL)-6, IL-1β, IL-18, tumor necroses factor (TNF)-α, interferon (IFN)-γ, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), 3-nitroturosine (3-NT), 4-hydroxynonenal (4-HNE) and cytokeratin 10 (K10) expression changes in MGs.Results: MGs showed plugging of orifice, glandular deficiency, abnormal acinar morphology, ductal dilatation, and lipid deposition after alkali burn. The expressions of IL-6, IL-18, IL-1β, IFN-γ, and TNF-α indicators of inflammation and oxidative stress in MGs tissues were significantly increased. Abnormal keratinization increased in the MG duct.Conclusion: A murine model of MGD is established by alkali burn of the eyelid margin that matches the clinical presentation of MGD providing a stable, short-time, low-cost, and reliable MGD model. The new method suggests efficient avenues for future research.","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"17 12","pages":"2158-2166"},"PeriodicalIF":1.9000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589446/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of ophthalmology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18240/ijo.2024.12.02","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD).

Methods: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model. The other eye was left untreated as a control group. Eyelid margin morphological changes and the meibomian glands (MGs) were observed by slit lamp microscopy on days 5 and 10 post-burn. Hematoxylin-eosin (HE) staining and Oil red O staining were adopted in detecting the changes in MGs morphology and lipid deposition. Real-time polymerase chain reaction, Western blot, immunofluorescence staining and immunohistochemical staining were used to detect interleukin (IL)-6, IL-1β, IL-18, tumor necroses factor (TNF)-α, interferon (IFN)-γ, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), 3-nitroturosine (3-NT), 4-hydroxynonenal (4-HNE) and cytokeratin 10 (K10) expression changes in MGs.

Results: MGs showed plugging of orifice, glandular deficiency, abnormal acinar morphology, ductal dilatation, and lipid deposition after alkali burn. The expressions of IL-6, IL-18, IL-1β, IFN-γ, and TNF-α indicators of inflammation and oxidative stress in MGs tissues were significantly increased. Abnormal keratinization increased in the MG duct.

Conclusion: A murine model of MGD is established by alkali burn of the eyelid margin that matches the clinical presentation of MGD providing a stable, short-time, low-cost, and reliable MGD model. The new method suggests efficient avenues for future research.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

碱烧伤小鼠睑板腺功能障碍模型。

目的：建立一种稳定、短时间、低成本、可靠的小鼠睑板腺功能障碍模型。方法：用滤纸蘸1.0 mol/L氢氧化钠（NaOH）溶液接触C57BL/6J小鼠眼睑缘10s建立模型。另一只眼睛不进行治疗，作为对照组。在烧伤后第5天和第10天，用裂隙灯显微镜观察眼睑边缘的形态学变化和睑板腺的变化。采用苏木精-伊红（HE）染色和油红O染色检测大鼠大鼠心肌形态和脂质沉积的变化。采用实时聚合酶链反应、Western blot、免疫荧光染色和免疫组织化学染色检测mg细胞中白细胞介素(IL)-6、IL-1β、IL-18、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶4 （NOX4）、3-硝基酪氨酸（3-NT）、4-羟基壬烯醛（4- hne）和细胞角蛋白10 （K10）的表达变化。结果：碱烧伤后，mg表现为孔口堵塞、腺体缺失、腺泡形态异常、导管扩张、脂质沉积。大鼠组织中炎症和氧化应激指标IL-6、IL-18、IL-1β、IFN-γ、TNF-α的表达均显著升高。MG管异常角化增加。结论：采用眼睑缘碱烧伤法建立的小鼠MGD模型符合MGD的临床表现，是一种稳定、短时间、低成本、可靠的MGD模型。这种新方法为今后的研究提供了有效的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

International journal of ophthalmology Medicine-Ophthalmology

CiteScore

2.50

自引率

7.10%

发文量

3141

审稿时长

4-8 weeks

期刊介绍： · International Journal of Ophthalmology-IJO (English edition) is a global ophthalmological scientific publication and a peer-reviewed open access periodical (ISSN 2222-3959 print, ISSN 2227-4898 online). This journal is sponsored by Chinese Medical Association Xi’an Branch and obtains guidance and support from WHO and ICO (International Council of Ophthalmology). It has been indexed in SCIE, PubMed, PubMed-Central, Chemical Abstracts, Scopus, EMBASE , and DOAJ. IJO JCR IF in 2017 is 1.166. IJO was established in 2008, with editorial office in Xi’an, China. It is a monthly publication. General Scientific Advisors include Prof. Hugh Taylor (President of ICO); Prof.Bruce Spivey (Immediate Past President of ICO); Prof.Mark Tso (Ex-Vice President of ICO) and Prof.Daiming Fan (Academician and Vice President, Chinese Academy of Engineering. International Scientific Advisors include Prof. Serge Resnikoff (WHO Senior Speciatist for Prevention of blindness), Prof. Chi-Chao Chan (National Eye Institute, USA) and Prof. Richard L Abbott (Ex-President of AAO/PAAO) et al. Honorary Editors-in-Chief: Prof. Li-Xin Xie(Academician of Chinese Academy of Engineering/Honorary President of Chinese Ophthalmological Society); Prof. Dennis Lam (President of APAO) and Prof. Xiao-Xin Li (Ex-President of Chinese Ophthalmological Society). Chief Editor: Prof. Xiu-Wen Hu (President of IJO Press). Editors-in-Chief: Prof. Yan-Nian Hui (Ex-Director, Eye Institute of Chinese PLA) and Prof. George Chiou (Founding chief editor of Journal of Ocular Pharmacology & Therapeutics). Associate Editors-in-Chief include: Prof. Ning-Li Wang (President Elect of APAO); Prof. Ke Yao (President of Chinese Ophthalmological Society) ; Prof.William Smiddy (Bascom Palmer Eye instituteUSA) ; Prof.Joel Schuman (President of Association of University Professors of Ophthalmology,USA); Prof.Yizhi Liu (Vice President of Chinese Ophtlalmology Society); Prof.Yu-Sheng Wang (Director of Eye Institute of Chinese PLA); Prof.Ling-Yun Cheng (Director of Ocular Pharmacology, Shiley Eye Center, USA). IJO accepts contributions in English from all over the world. It includes mainly original articles and review articles, both basic and clinical papers. Instruction is Welcome Contribution is Welcome Citation is Welcome Cooperation organization International Council of Ophthalmology(ICO), PubMed, PMC, American Academy of Ophthalmology, Asia-Pacific, Thomson Reuters, The Charlesworth Group, Crossref,Scopus,Publons, DOAJ etc.

期刊最新文献

CORRIGENDUM. CORRIGENDUM. COVID-19's effects on microvascular structure in a healthy retina: an OCTA study. Efficacy and safety of combined Kahook Dual Blade goniotomy with phacoemulsification in Chinese patients with primary open angle glaucoma. Idiopathic uveal effusion syndrome with increased intraocular pressure.