An analysis of the structural changes of the oxygen evolving complex of Photosystem II in the S1 and S3 states revealed by serial femtosecond crystallography

IF 3.4 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochimica et Biophysica Acta-Bioenergetics Pub Date : 2024-12-16 DOI:10.1016/j.bbabio.2024.149531
Salma Yehia , Jimin Wang , Gary W. Brudvig , M.R. Gunner , Bernard R. Brooks , Muhamed Amin
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Abstract

Photosystem II (PSII) is a unique natural catalyst that converts solar energy into chemical energy using earth abundant elements in water at physiological pH. Understanding the reaction mechanism will aid the design of biomimetic artificial catalysts for efficient solar energy conversion. The Mn4O5Ca cluster cycles through five increasingly oxidized intermediates before oxidizing two water molecules into O2 and releasing protons to the lumen and electrons to drive PSII reactions. The Mn coordination and OEC electronic structure changes through these intermediates. Thus, obtaining a high-resolution structure of each catalytic intermediate would help reveal the reaction mechanism. While valuable structural information was obtained from conventional X-ray crystallography, time-resolution of conventional X-ray crystallography limits the analysis of shorted-lived reaction intermediates. Serial Femtosecond X-ray crystallography (SFX), which overcomes the radiation damage by using ultra short laser pulse for imaging, has been used extensively to study the water splitting intermediates in PSII. Here, we review the state of the art and our understanding of the water splitting reaction before and after the advent of SFX. Furthermore, we analyze the likely Mn coordination in multiple XFEL structures prepared in the dark-adapted S1 state and those following two-flashes which are poised in the penultimate S3 oxidation state based on Mn coordination chemistry. Finally, we summarize the major contributions of the SFX to our understanding of the structures of the S1 and S3 states.
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用连续飞秒晶体学分析光系统II的S1和S3态析氧配合物的结构变化。
光系统II (Photosystem II, PSII)是一种独特的天然催化剂,利用水中富土元素在生理ph下将太阳能转化为化学能,了解其反应机理将有助于设计高效太阳能转化的仿生人工催化剂。Mn4O5Ca簇通过5个日益氧化的中间体循环,然后将两个水分子氧化成O2,并向管腔释放质子和电子来驱动PSII反应。Mn配位和OEC电子结构通过这些中间体发生改变。因此,获得每个催化中间体的高分辨率结构将有助于揭示反应机理。虽然从传统的x射线晶体学中获得了有价值的结构信息,但传统x射线晶体学的时间分辨率限制了对短寿命反应中间体的分析。连续飞秒x射线晶体学(SFX)利用超短激光脉冲成像克服了辐射损伤,被广泛用于研究PSII中水分裂中间体。在这里,我们回顾了SFX出现前后的艺术状态和我们对水分解反应的理解。此外,基于Mn配位化学,我们分析了在适应黑暗的S1态和两次闪光后处于倒数第二S3氧化态的多个XFEL结构中可能的Mn配位。最后,我们总结了SFX对我们理解S1和S3态结构的主要贡献。
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来源期刊
Biochimica et Biophysica Acta-Bioenergetics
Biochimica et Biophysica Acta-Bioenergetics 生物-生化与分子生物学
CiteScore
9.50
自引率
7.00%
发文量
363
审稿时长
92 days
期刊介绍: BBA Bioenergetics covers the area of biological membranes involved in energy transfer and conversion. In particular, it focuses on the structures obtained by X-ray crystallography and other approaches, and molecular mechanisms of the components of photosynthesis, mitochondrial and bacterial respiration, oxidative phosphorylation, motility and transport. It spans applications of structural biology, molecular modeling, spectroscopy and biophysics in these systems, through bioenergetic aspects of mitochondrial biology including biomedicine aspects of energy metabolism in mitochondrial disorders, neurodegenerative diseases like Parkinson''s and Alzheimer''s, aging, diabetes and even cancer.
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