Uneven Distribution of Purkinje Cell Injury in the Cerebellar Vermis of Term Neonates with Hypoxic-Ischemic Encephalopathy.

IF 2.7 3区 医学 Q3 NEUROSCIENCES Cerebellum Pub Date : 2024-12-19 DOI:10.1007/s12311-024-01765-z
Kim V Annink, Ilona C E van Leeuwen, Nina A Smeets, Lianne A J Peeters, Niek E van der Aa, Thomas Alderliesten, Floris Groenendaal, Reint K Jellema, Cora H A Nijboer, Peter G J Nikkels, Martin Lammens, Manon J N L Benders, Freek E Hoebeek, Jeroen Dudink
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Abstract

In term neonates with hypoxic-ischemic encephalopathy (HIE), cerebellar injury is becoming more and more acknowledged. Animal studies demonstrated that Purkinje cells (PCs) are especially vulnerable for hypoxic-ischemic injury. In neonates, however, the extent and pattern of PC injury has not been investigated. The aim of this study was to characterize the morphology and distribution of PCs in the cerebellar vermis of term born neonates with HIE. Twenty-two term born neonates with severe HIE, several of which received therapeutic hypothermia, who underwent post-mortem autopsy of the brain including cerebellar vermis within three to five days after birth were included. Haematoxylin & Eosin (H&E) stained sections of the vermis were used to determine total PC count and morphology (normal, abnormal or non-classified) at the bases and crowns of the folia and of the lobules in both the anterior and posterior lobes. Differences in PC count and PC morphology between the anterior and posterior lobe and between the bases and crowns were compared. The total number of PCs was significantly higher at the crowns compared to the bases (p < 0.001) irrespective of the precise location. Besides, PCs at the bases more often had an abnormal morphology. Also, a significant difference between the injury in the anterior and posterior lobe was observed, notably at specific microscopic locations with more abnormal PCs in the posterior lobe. The number of PCs scored as abnormal was increased in the bases compared to the crowns, which might resemble supratentorial ulegyria.

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缺氧缺血性脑病足月新生儿小脑蚓浦肯野细胞损伤分布不均匀。
在足月新生儿缺氧缺血性脑病(HIE)中,小脑损伤越来越受到重视。动物实验表明,浦肯野细胞(PCs)特别容易受到缺氧缺血性损伤。然而,在新生儿中,PC损伤的程度和模式尚未被调查。本研究的目的是表征足月新生儿HIE小脑蚓部PCs的形态和分布。22名患有严重HIE的足月新生儿,其中一些接受了治疗性低温治疗,他们在出生后3至5天内对大脑进行了尸检,包括小脑蚓。使用红木精和伊红(H&E)染色的蚓部切片来测定叶基部和叶顶以及前后叶小叶的PC总数和形态学(正常、异常或未分类)。比较前叶与后叶、基托与冠间PC计数和形态的差异。树冠处的pc总数显著高于基部(p
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来源期刊
Cerebellum
Cerebellum 医学-神经科学
CiteScore
6.40
自引率
14.30%
发文量
150
审稿时长
4-8 weeks
期刊介绍: Official publication of the Society for Research on the Cerebellum devoted to genetics of cerebellar ataxias, role of cerebellum in motor control and cognitive function, and amid an ageing population, diseases associated with cerebellar dysfunction. The Cerebellum is a central source for the latest developments in fundamental neurosciences including molecular and cellular biology; behavioural neurosciences and neurochemistry; genetics; fundamental and clinical neurophysiology; neurology and neuropathology; cognition and neuroimaging. The Cerebellum benefits neuroscientists in molecular and cellular biology; neurophysiologists; researchers in neurotransmission; neurologists; radiologists; paediatricians; neuropsychologists; students of neurology and psychiatry and others.
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