Bashar Altakroni, Helen Hunter, Greg Horne, Daniel R Brison, Andrew C Povey
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引用次数: 0
Abstract
Sperm DNA contains strand breaks and base damage that can potentially affect reproductive health. This study aims to determine to what extent sperm DNA integrity and alkylation is associated with semen quality and assisted reproduction technology (ART) treatment outcomes, in particular fertilisation and cleavage rates. Male partners of couples attending for infertility treatment were recruited. DNA integrity (% tail DNA, sperm with either low (LDD) or high (HDD) damage levels) was measured by a neutral Comet assay and N7-methyldeoxyguanosine (N7-MedG) DNA levels by an immunoslotblot in sperm prepared by density gradient centrifugation. Associations between DNA damage, semen quality and ART treatment outcomes were assessed. N7-MedG levels were lower and the proportion of LDD sperm higher in prepared than in neat sperm samples. The proportion of HDD sperm and % tail DNA were significantly negatively associated and the proportion of LDD sperm positively associated with semen quality. Fertilisation, but not cleavage, rate nor live birth, was significantly negatively associated with N7-MedG levels, the proportion of HDD sperm and % tail DNA and was positively associated with the proportion of LDD sperm. These results confirm that DNA damage, even in prepared sperm, is associated with adverse semen quality and suggest that sperm DNA damage affects the early stages of embryo formation.
精子 DNA 含有可能影响生殖健康的链断裂和碱基损伤。本研究旨在确定精子 DNA 的完整性和烷基化在多大程度上与精液质量和辅助生殖技术(ART)治疗效果(尤其是受精率和卵裂率)相关。研究人员招募了接受不孕不育治疗的夫妇的男性伴侣。DNA完整性(尾部DNA百分比、低(LDD)或高(HDD)损伤水平的精子)通过中性彗星试验进行测量,N7-甲基脱氧鸟苷(N7-MedG)DNA水平通过密度梯度离心法制备的精子中的免疫印迹进行测量。评估了DNA损伤、精液质量和抗逆转录病毒疗法治疗结果之间的关联。在制备好的精子样本中,N7-MedG水平低于纯精子样本,LDD精子比例高于纯精子样本。HDD精子比例和尾部DNA%与精液质量呈显著负相关,而LDD精子比例与精液质量呈正相关。受精率与 N7-MedG 水平、HDD 精子比例和尾部 DNA 百分比呈显著负相关,而与 LDD 精子比例呈正相关,但与卵裂率和活产率无关。这些结果证实,DNA损伤(即使是在制备好的精子中)与精液质量不良有关,并表明精子 DNA 损伤会影响胚胎形成的早期阶段。
期刊介绍:
Human Fertility is a leading international, multidisciplinary journal dedicated to furthering research and promoting good practice in the areas of human fertility and infertility. Topics included span the range from molecular medicine to healthcare delivery, and contributions are welcomed from professionals and academics from the spectrum of disciplines concerned with human fertility. It is published on behalf of the British Fertility Society.
The journal also provides a forum for the publication of peer-reviewed articles arising out of the activities of the Association of Biomedical Andrologists, the Association of Clinical Embryologists, the Association of Irish Clinical Embryologists, the British Andrology Society, the British Infertility Counselling Association, the Irish Fertility Society and the Royal College of Nursing Fertility Nurses Group.
All submissions are welcome. Articles considered include original papers, reviews, policy statements, commentaries, debates, correspondence, and reports of sessions at meetings. The journal also publishes refereed abstracts from the meetings of the constituent organizations.