EFFECTS OF REAGENTS AND CHEMICALS USED IN EXISTING SANITATION HELMINTH TEST METHODS ON THE VIABILITY OF ASCARIS SUUM EGGS.

IF 1 4区 医学 Q4 PARASITOLOGY Journal of Parasitology Pub Date : 2024-12-01 DOI:10.1645/22-58
D Naidoo, C E Archer
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Abstract

Various sanitation methods to recover helminth eggs are currently in use; however, no international standard exists. Development of such a method first involves testing the effects of all reagents used in current methods on helminth egg viability to determine whether these chemicals affect the test organism. This study was conducted to investigate the effects on viability and development of Ascaris suum eggs when exposed for various periods to wash solutions (water, ammonium bicarbonate, Tween® 20, Tween® 80, Triton® X-100, Sunlight® Liquid, bentonite, and 7X®), flotation solutions (zinc sulfate, magnesium sulfate, sodium nitrate, brine, and sucrose), extraction solutions (10% formalin, acetoacetic buffer, acid-alcohol, ethyl acetate, and diethyl ether), extraction combinations (10% formalin + ethyl acetate, 10% formalin + diethyl ether, acetoacetic buffer + ethyl acetate, acetoacetic buffer + diethyl ether, and acid-alcohol + ethyl acetate), and incubation solutions (water, 0.1 N sulfuric acid, physiological saline, and 0.5%, 2%, and 5% formalin). Ammonium bicarbonate and 7X® performed best as wash solutions (including for overnight soaking), and zinc sulfate is recommended for flotation for up to 30 min of exposure. Individually, all extraction solutions had minimal effects on egg viability, and in combination, acid-alcohol and ethyl acetate did not impact egg viability for up to 15 min of exposure. Postincubation, sulfuric acid allowed optimal egg development and clear samples for microscopy.

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现有卫生蠕虫检验方法中使用的试剂和化学品对蛔虫卵活力的影响。
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来源期刊
Journal of Parasitology
Journal of Parasitology 医学-寄生虫学
CiteScore
2.10
自引率
7.70%
发文量
60
审稿时长
2 months
期刊介绍: The Journal of Parasitology is the official peer-reviewed journal of the American Society of Parasitologists (ASP). The journal publishes original research covering helminths, protozoa, and other parasitic organisms and serves scientific professionals in microbiology, immunology, veterinary science, pathology, and public health. Journal content includes original research articles, brief research notes, announcements of the Society, and book reviews. Articles are subdivided by topic for ease of reference and range from behavior and pathogenesis to systematics and epidemiology. The journal is published continuously online with one full volume printed at the end of each year.
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