How the Magenstrasse Is Formed After Meals: Protein Aggregation Hypothesis.

IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Journal of pharmaceutical sciences Pub Date : 2024-12-17 DOI:10.1016/j.xphs.2024.12.008
Ryosuke Sakai, Yoshiyuki Shirasaka, Taiyo Takagi, Takato Masada, Keiko Minami, Makoto Kataoka, Ikumi Tamai, Toshihide Takagi, Shinji Yamashita
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Abstract

Magenstrasse (stomach road) is reported to potentially influence the absorption of orally administered drugs by facilitating a gastric emptying of ingested water under postprandial condition. We hypothesized the Magenstrasse is a consequence of the formation of protein aggregates due to the decrease in gastric pH associated with stimulated gastric acid secretion. The formation mechanism of the Magenstrasse was examined in vitro using a gastric chamber system which reproduces postprandial conditions in the stomach. Oral liquid meals containing different amounts of proteins were mixed with simulated gastric fluid containing pepsin in the gastric chamber. When a high-protein liquid meal was used, infusion of gastric acid caused protein denaturation, generating semisolid aggregates. Then, to evaluate the impact of the aggregates, fluorescein isothiocyanate-dextran 4000 (FD-4) solution was added. The presence of protein aggregates facilitated the elution of FD-4 from the gastric chamber, indicating that the semisolid aggregates suppressed mixing of FD-4 solution with meals. In addition, formation of the same type of protein aggregates was observed in vivo in rat stomach after ingesting a high-protein liquid meal. These in vitro and in vivo results support the idea that protein aggregation of liquid meals in the stomach contributes the formation of the Magenstrasse.

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据报道,在餐后状态下,胃粘膜(胃路)可促进摄入水分的胃排空,从而影响口服药物的吸收。我们推测,马根斯特拉斯是由于胃酸分泌刺激导致胃 pH 值降低而形成蛋白质聚集体的结果。我们使用胃室系统对马根路斯的形成机制进行了体外研究,该系统再现了餐后胃中的情况。在胃室中将含有不同蛋白质量的口服流质膳食与含有胃蛋白酶的模拟胃液混合。当使用高蛋白流质膳食时,注入的胃酸会导致蛋白质变性,生成半固体聚集体。然后,为了评估聚集体的影响,加入了异硫氰酸荧光素-葡聚糖 4000(FD-4)溶液。蛋白质聚集体的存在促进了 FD-4 从胃腔中的洗脱,表明半固体聚集体抑制了 FD-4 溶液与膳食的混合。此外,在大鼠胃中摄入高蛋白流质膳食后,也观察到体内形成了相同类型的蛋白质聚集体。这些体外和体内研究结果都支持这样一种观点,即胃中的流质膳食蛋白质聚集有助于马根氏菌的形成。
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来源期刊
CiteScore
7.30
自引率
13.20%
发文量
367
审稿时长
33 days
期刊介绍: The Journal of Pharmaceutical Sciences will publish original research papers, original research notes, invited topical reviews (including Minireviews), and editorial commentary and news. The area of focus shall be concepts in basic pharmaceutical science and such topics as chemical processing of pharmaceuticals, including crystallization, lyophilization, chemical stability of drugs, pharmacokinetics, biopharmaceutics, pharmacodynamics, pro-drug developments, metabolic disposition of bioactive agents, dosage form design, protein-peptide chemistry and biotechnology specifically as these relate to pharmaceutical technology, and targeted drug delivery.
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