Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Letters in Applied Microbiology Pub Date : 2025-01-06 DOI:10.1093/lambio/ovae135

Shubham Mathur, Ikram Salah, Haneen Msarwa, Hillary A Craddock, Jacob Moran-Gilad

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Abstract

This study evaluated a point-of-care device for wastewater-based epidemiology (WBE) of SARS-CoV-2 circulation. WBE is a critical tool but faces challenges such as laboratory infrastructure, sample transport, skilled personnel, and time-consuming methods. Currently, a rapid, portable solution for wastewater surveillance is lacking. From June to September 2022, the performance of the GeneXpert instrument was compared with an established in-house method for detecting SARS-CoV-2 in 151 wastewater samples across laboratory, field, and mobile testing environments. In the laboratory setting, GeneXpert showed 100% agreement in detection, with 94.6% of positive samples displaying a viral load difference of ± 1 log compared to the in-house method. Field testing demonstrated 90% agreement, with 92.6% of GeneXpert-positive samples showing similar log differences. In mobile testing, GeneXpert detected 62.5% of samples, and testing faced technical issues. Overall, GeneXpert displayed a limit of detection of 12 cpml-1 with results available in under an hour, making it a promising tool for rapid and deployable wastewater testing. This requires further validation while addressing technical challenges related to field and mobile settings.

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利用移动实验室检测和定量废水中的 SARS-CoV-2

本研究评估了一种用于SARS-CoV-2废水流行病学（WBE）循环的护理点（POC）装置。基于废水的流行病学是一种关键工具，但面临实验室基础设施、样本运输、熟练人员和耗时方法等挑战。目前，缺乏一种快速、便携的废水监测解决方案。从2022年6月至9月，将GeneXpert仪器的性能与在实验室、现场和移动测试环境中检测151份废水样品中SARS-CoV-2的既定内部方法进行了比较。在实验室环境中，GeneXpert在检测中显示100%的一致性，与内部方法相比，94.6%的阳性样本显示病毒载量差异为±1 Log。现场测试显示90%的一致性，92.6%的genexpert阳性样本显示相似的Log差异。在移动测试中，GeneXpert检测到62.5%的样本，测试面临技术问题。总的来说，GeneXpert显示出12 cpml-1的检测限，并在一小时内获得结果，使其成为一种有前途的快速部署废水测试工具。这需要进一步验证，同时解决与现场和移动设置相关的技术挑战。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.