Role of forelimb morphology in muscle sensorimotor functions during locomotion in the cat.

Abstract

Previous studies established strong links between morphological characteristics of mammalian hindlimb muscles and their sensorimotor functions during locomotion. Less is known about the role of forelimb morphology in motor outputs and generation of sensory signals. Here, we measured morphological characteristics of 46 forelimb muscles from six cats. These characteristics included muscle attachments, physiological cross-sectional area (PCSA) and fascicle length. We also recorded full-body mechanics and EMG activity of forelimb muscles during level overground and treadmill locomotion in seven and 16 adult cats of either sex, respectively. We computed forelimb muscle forces along with force- and length-dependent sensory signals mapped onto corresponding cervical spinal segments. We found that patterns of computed muscle forces and afferent activities were strongly affected by the muscle's moment arm, PCSA and fascicle length. Morphology of the shoulder muscles suggests distinct roles of the forelimbs in lateral force production and movements. Patterns of length-dependent sensory activity of muscles with long fibres (brachioradialis, extensor carpi radialis) closely matched patterns of overall forelimb length, whereas the activity pattern of biceps brachii length afferents matched forelimb orientation. We conclude that cat forelimb muscle morphology contributes substantially to locomotor function, particularly to control lateral stability and turning, rather than propulsion. KEY POINTS: Little is known about the role of forelimb muscle morphology in producing motor outputs and generating somatosensory signals. This information is needed to understand the contributions of forelimbs in locomotor control. We measured morphological characteristics of 46 muscles from cat forelimbs, recorded cat walking mechanics and electromyographic activity, and computed patterns of moment arms, length, velocity, activation, and force of forelimb muscles, as well as length- and force-dependent afferent activity during walking. We demonstrated that moment arms, physiological cross-sectional area and fascicle length of forelimb muscles contribute substantially to muscle force production and proprioceptive activity, to the regulation of locomotor cycle phase transitions and to control of lateral stability. The obtained information can guide the development of biologically accurate neuromechanical models of quadrupedal locomotion for exploring and testing novel methods of treatments of central nervous system pathologies by modulating activities in neural pathways controlling forelimbs/arms.