Biochemical and genetic studies of bacteria metabolizing lignin-related compounds.

Abstract

The ability of bacterial strains to metabolize lignin model compounds was studied. Strains examined were non-filamentous bacterial isolates obtained from decaying wood and the actinomycete Streptomyces viridosporus T7A. Model compounds included dimers containing either the beta-1 (1,2-diarylethane) or the beta-O-4 (arylglycerol-beta-aryl ether) type of linkage. Pseudomonas fluorescens biovar I A1 proliferated on anisoin (4,4'-dimethoxybenzoin) accumulating anisic acid temporarily. Cleavage at the beta-1 bond was also observed with crude extracts prepared from the same strain. In turn, cleavage of the beta-O-4 linkage of veratrylglycerol-beta-guaiacyl ether was detected in cultures of Pseudomonas acidovorans D3. In this case, main degradation intermediates were beta-hydroxypropioveratrone, acetoveratrone and guaiacol. S. viridosporus T7A reduced the carbonyl group of some beta-1 dimers and did not modify the beta-O-4 model compounds tested. Attempts to ascribe a catabolic character to large molecular weight extrachromosomal DNA present in some strains were unsuccessful. Gene banks of P. fluorescens biovar I A1 and P. acidovorans D3 were prepared utilizing the broad host range cosmid pLAFR1 as vector.