Spontaneous and salt stress-induced molecular instability in the progeny of MSH7 deficient Arabidopsis thaliana plants

Abstract

The MSH7 protein is a binding partner of MSH2 forming the MutSγ complex. This complex contributes to the plant mismatch repair (MMR) system by recognizing DNA base-base mismatches. Here, we evaluated the impact of MSH7 on genetic diversity of the tenth generation (G₁₀) of wild type and MSH7 deficient Arabidopsis thaliana plants before and after two days exposure to 100 mM NaCl. Genetic diversity was assessed using inter simple sequence repeats (ISSR) and high-resolution melting (HRM) analyses. ISSR analyses revealed a 6.7 % or 5.8 % average polymorphism in the G₁₀ of wild type before and after a short-term salt stress, respectively, and a 64.4 % or 72.1 % average polymorphism in the G₁₀ of msh7 mutant plants before and after salt treatment, respectively. Interestingly, several ISSR markers showed different polymorphism patterns after salt stress compared with the control before treatment. We next compared the percentage of the G₁₀ of wild type and msh7 seedlings with polymorphic bands. Statistically significant differences between genotypes but not due to the salt treatment were observed. In addition, co-amplification at lower temperature-PCR followed by HRM analysis was performed. Of the five assayed HRM loci, two loci allowed the discrimination of fragment alleles between genotypes and two loci, between conditions. We conclude that MSH7 deficient A. thaliana mutants accumulated mutations over 10 generations, and that two days of salt stress caused a further increase in new mutations, thus enhancing genetic diversity that may favor new traits associated with stress tolerance, fitness, and adaptation.