Epigenetic Regulation by Histone Methylation and Demethylation in Freeze-Tolerant Frog Kidney

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Biochemistry and Function Pub Date : 2024-12-20 DOI:10.1002/cbf.70036

Olawale O. Taiwo, Sarah A. Breedon, Kenneth B. Storey

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Abstract

The wood frog (Rana sylvatica) endures whole-body freezing over the winter, with extensive extracellular ice formation and halted physiological activities. Epigenetic mechanisms, including reversible histone lysine methylation, enable quick alterations in gene expression, helping to maintain viability during freeze-thaw cycles. The present study evaluated eight histone lysine methyltransferases (KMTs), 10 histone lysine demethylases (KDMs), and 11 histone marks in wood frog kidneys. Using immunoblotting, significant changes in relative protein levels of multiple KMTs and KDMs were observed in response to freezing, with variable alterations during thawing. Specifically, the repressive methyl marks H3K27me1 and H4K20me3 significantly decreased during freezing, whereas H3K9me3, H3K27me3, and H3K36me2 decreased during thawing. These results demonstrate that the regulation of histone methylation and demethylation play crucial roles in controlling gene expression over the freeze-thaw cycle and the maintenance of normal renal physiology.

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耐冻蛙肾中组蛋白甲基化和去甲基化的表观遗传调控

林蛙（Rana sylvatica）在冬季经受全身冰冻，细胞外形成大量冰层，生理活动停止。表观遗传机制（包括可逆的组蛋白赖氨酸甲基化）可快速改变基因表达，帮助在冻融循环中维持生命力。本研究评估了林蛙肾脏中的8种组蛋白赖氨酸甲基转移酶（KMTs）、10种组蛋白赖氨酸去甲基化酶（KDMs）和11种组蛋白标记。通过免疫印迹法，观察到多种 KMTs 和 KDMs 的相对蛋白水平在冷冻时发生了显著变化，在解冻时也有不同程度的变化。具体来说，抑制性甲基标记 H3K27me1 和 H4K20me3 在冷冻过程中显著下降，而 H3K9me3、H3K27me3 和 H3K36me2 则在解冻过程中下降。这些结果表明，组蛋白甲基化和去甲基化的调控在控制冻融周期中的基因表达和维持正常肾脏生理功能方面起着至关重要的作用。

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来源期刊

Cell Biochemistry and Function 生物-生化与分子生物学

CiteScore

6.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Cell Biochemistry and Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease. The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies of cells in situ are especially encouraged. Observational and pathological studies will be considered providing they include a rational discussion of the possible molecular and biochemical mechanisms behind them and the immediate impact of these observations to our understanding of mammalian biology.