Metabologenomics-Driven Discovery of Nocardimicins from a Psychrophilic Nocardia sp. Strain.

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL Journal of Natural Products Pub Date : 2024-12-20 DOI:10.1021/acs.jnatprod.4c01140

Suling Xu, Nengfei Wang, Qingzhou Meng, Wenjie Ma, Huayue Li

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引用次数: 0

Abstract

A combined strategy of 2D-NMR-metabolomics-driven substructure tracking with genome mining led to the targeted discovery of 10 nocobactin-type lipopeptides (1-10) from the Arctic-derived phychrophillic Nocardia sp. L-016, among which 1-5 are new compounds, named nocardimicins S-W. The phenoxazole moiety in 1-10, featuring unique NMR values and correlations, was used as a probe for tracking nocardimicin analogues. The structures of 1-5 were established based on extensive MS and NMR spectroscopic analyses. The biosynthesis of nocardimicins (1-10) in Nocardia sp. L-016 is proposed to be achieved by the noc biosynthetic gene cluster, which is composed of two sub-gene clusters (I and II) separated by a 228 kb region. Compounds 1-10 showed moderate inhibition against human cancer cell lines of HCT116 and HepG2 with IC₅₀ values in the range of 3.5-10.2 μM. This work provides an effective application of paired-omics technologies in the discovery of new natural products.

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.