Neuroprotective effect of red dragon fruit extract ameliorates oxidative stress and inflammation in D-galactose-induced aging rat model: biochemical, histological and immunohistochemical study

IF 2.9 4区生物学 Q3 CELL BIOLOGY Journal of Molecular Histology Pub Date : 2024-12-20 DOI:10.1007/s10735-024-10316-z

Manar Fouli Gaber Ibrahim, Fatma F. Ali, Sayed Fouad El-Sheikh Ali, Emad S. Shaker, Hemdan I. Mahmoud, Fatma ElZahraa Mohammed Abdellatif, Sahar A. Mokhemer

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Abstract

Aging is a worldwide socioeconomic burden. Cerebellar aging is an enigma contributing to many behavioral aging disorders, hence is its hindering by prophylactic measurements is a crucial geriatric research target. Red dragon fruit (RDF) is a tropical fruit with antioxidant, anti-inflammatory and anti-apoptotic properties. This study aimed to determine the protective effect of RDF extract against cerebellar aging. Thirty-two male albino rats were randomly allocated into 4 groups: Control, RDF, aged and RDF-aged groups. Aged group revealed structural distortion affecting cerebellar layers including a significant (P < 0.05) decrease in Purkinje cells number and decrease in granular cell layer thickness by comparison to the control and RDF groups. Additionally, distorted capillary endothelium, and defective myelination were noticed. Interestingly, cerebellar active caspase-3, iNOS, MDA and 3-NT and serum TNF-α levels significantly increased with aging by comparison to the control and RDF groups (all P < 0.05). Biochemical analysis revealed a significant (P < 0.05) decrease in cerebellar SOD and serum GSH levels in aged rats. RDF extract remarkably ameliorated most of the neuronal degenerative changes with a significant (P < 0.05) increase in Purkinje cells numbers, and granular cell layer thickness by comparison to the aged group. Furthermore, it resulted in a significant (P < 0.05) decrease in cerebellum expression of active caspase-3, iNOS, MDA, 3-NT, and serum TNF-α levels associated with a significant (P < 0.05) increase in cerebellar SOD and serum GSH levels by comparison to the aged group. To the best of our knowledge this is the first study showing a neuroprotective effect for RDF against cerebellar aging. RDF might be effective in attenuation of age-induced cerebellar degenerative changes through its anti-apoptotic, antioxidant and anti-inflammatory effects.

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红火果提取物改善d -半乳糖诱导衰老大鼠氧化应激和炎症的神经保护作用：生化、组织学和免疫组织化学研究

老龄化是一个全球性的社会经济负担。小脑老化是导致许多行为老化障碍的一个谜，因此如何通过预防措施来阻止小脑老化是老年病学研究的一个重要目标。红火龙果（RDF）是一种具有抗氧化、抗炎和抗细胞凋亡特性的热带水果。本研究旨在探讨RDF提取物对小脑衰老的保护作用。将32只雄性白化大鼠随机分为4组：对照组、RDF组、老年组和RDF老龄组。与对照组和RDF组相比，老龄组小脑层结构扭曲，浦肯野细胞数量和颗粒细胞层厚度显著减少（P < 0.05）。毛细血管内皮扭曲，髓鞘形成缺陷。有趣的是，与对照组和RDF组相比，随着年龄的增长，小脑活性caspase-3、iNOS、MDA、3-NT和血清TNF-α水平显著升高（P < 0.05）。生化分析显示老龄大鼠小脑SOD和血清GSH水平显著（P < 0.05）降低。与老年组相比，RDF提取物显著改善了大部分神经元退行性改变，浦肯野细胞数量和颗粒细胞层厚度显著（P < 0.05）增加。此外，与老年组相比，大鼠小脑活性caspase-3、iNOS、MDA、3-NT和血清TNF-α表达显著（P < 0.05）降低，小脑SOD和血清GSH水平显著（P < 0.05）升高。据我们所知，这是第一个显示RDF对小脑老化具有神经保护作用的研究。RDF可能通过其抗凋亡、抗氧化和抗炎作用，有效抑制老年性小脑退行性改变。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.